SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Akre Olof)
 

Search: WFRF:(Akre Olof) > Quantity and qualit...

  • Carlsson, Jessica,1984-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Urology (author)

Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies

  • Article/chapterEnglish2018

Publisher, publication year, extent ...

  • 2018-12-05
  • BioMed Central,2018
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:oru-70618
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-70618URI
  • https://doi.org/10.1186/s12874-018-0628-1DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:139798202URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding Agencies:Italian Association for Cancer Research (IARC)  Örebro county council research committee  Lions cancer research foundation in Uppsala/Örebro 
  • BACKGROUND: In Sweden, human tissue samples obtained from diagnostic and surgical procedures have for decades been routinely stored in a formalin-fixed, paraffin-embedded, form. Through linkage with nationwide registers, these samples are available for molecular studies to identify biomarkers predicting mortality even in slow-progressing prostate cancer. However, tissue fixation causes modifications of nucleic acids, making it challenging to extract high-quality nucleic acids from formalin fixated tissues.METHODS: In this study, the efficiency of five commercial nucleic acid extraction kits was compared on 30 prostate biopsies with normal histology, and the quantity and quality of the products were compared using spectrophotometry and Agilent's BioAnalyzer. Student's t-test's and Bland-Altman analyses were performed in order to investigate differences in nucleic acid quantity and quality between the five kits. The best performing extraction kits were subsequently tested on an additional 84 prostate tumor tissues. A Spearman's correlation test and linear regression analyses were performed in order to investigate the impact of tissue age and amount of tissue on nucleic acid quantity and quality.RESULTS: Nucleic acids extracted with RNeasy® FFPE and QIAamp® DNA FFPE Tissue kit had the highest quantity and quality, and was used for extraction from 84 tumor tissues. Nucleic acids were successfully extracted from all biopsies, and the amount of tumor (in millimeter) was found to have the strongest association with quantity and quality of nucleic acids.CONCLUSIONS: To conclude, this study shows that the choice of nucleic acid extraction kit affects the quantity and quality of extracted products. Furthermore, we show that extraction of nucleic acids from archival formalin-fixed prostate biopsies is possible, allowing molecular studies to be performed on this valuable sample collection.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Davidsson, Sabina,1972-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Urology, Faculty of Medicine and Health, University Hospital, Örebro, Sweden(Swepub:oru)sdn (author)
  • Fridfeldt, JonnaDepartment of Urology, Faculty of Medicine and Health, University Hospital Örebro, Örebro, Sweden; Örebro University, Örebro, Sweden (author)
  • Giunchi, FrancescaDepartment of Pathology, F. Addari Institute of Oncology S. Orsola Hospital, Bologna, Italy (author)
  • Fiano, ValentinaCancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy (author)
  • Grasso, ChiaraCancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy (author)
  • Zelic, RenataKarolinska Institutet (author)
  • Richiardi, LorenzoCancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy (author)
  • Andrén, Ove,1963-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Urology, Faculty of Medicine and Health, University Hospital Örebro, Örebro, Sweden(Swepub:oru)oan (author)
  • Pettersson, AndreasKarolinska Institutet (author)
  • Fiorentino, MichelangeloDepartment of Pathology, F. Addari Institute of Oncology S. Orsola Hospital, Bologna, Italy (author)
  • Akre, OlofKarolinska Institutet (author)
  • Örebro universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

Related titles

  • In:BMC Medical Research Methodology: BioMed Central18:11471-2288

Internet link

Find in a library

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view