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Quantity and qualit...
Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies
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- Carlsson, Jessica, 1984- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Urology
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- Davidsson, Sabina, 1972- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Urology, Faculty of Medicine and Health, University Hospital, Örebro, Sweden
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- Fridfeldt, Jonna (författare)
- Department of Urology, Faculty of Medicine and Health, University Hospital Örebro, Örebro, Sweden; Örebro University, Örebro, Sweden
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- Giunchi, Francesca (författare)
- Department of Pathology, F. Addari Institute of Oncology S. Orsola Hospital, Bologna, Italy
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- Fiano, Valentina (författare)
- Cancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy
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- Grasso, Chiara (författare)
- Cancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy
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- Zelic, Renata (författare)
- Karolinska Institutet
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- Richiardi, Lorenzo (författare)
- Cancer Epidemiology Unit-CERMS, Department of Medical Sciences, University of Turin and CPO-Piemonte, Turin, Italy
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- Andrén, Ove, 1963- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Urology, Faculty of Medicine and Health, University Hospital Örebro, Örebro, Sweden
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- Pettersson, Andreas (författare)
- Karolinska Institutet
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- Fiorentino, Michelangelo (författare)
- Department of Pathology, F. Addari Institute of Oncology S. Orsola Hospital, Bologna, Italy
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- Akre, Olof (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2018-12-05
- 2018
- Engelska.
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Ingår i: BMC Medical Research Methodology. - : BioMed Central. - 1471-2288. ; 18:1
- Relaterad länk:
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https://doi.org/10.1...
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https://bmcmedresmet...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- BACKGROUND: In Sweden, human tissue samples obtained from diagnostic and surgical procedures have for decades been routinely stored in a formalin-fixed, paraffin-embedded, form. Through linkage with nationwide registers, these samples are available for molecular studies to identify biomarkers predicting mortality even in slow-progressing prostate cancer. However, tissue fixation causes modifications of nucleic acids, making it challenging to extract high-quality nucleic acids from formalin fixated tissues.METHODS: In this study, the efficiency of five commercial nucleic acid extraction kits was compared on 30 prostate biopsies with normal histology, and the quantity and quality of the products were compared using spectrophotometry and Agilent's BioAnalyzer. Student's t-test's and Bland-Altman analyses were performed in order to investigate differences in nucleic acid quantity and quality between the five kits. The best performing extraction kits were subsequently tested on an additional 84 prostate tumor tissues. A Spearman's correlation test and linear regression analyses were performed in order to investigate the impact of tissue age and amount of tissue on nucleic acid quantity and quality.RESULTS: Nucleic acids extracted with RNeasy® FFPE and QIAamp® DNA FFPE Tissue kit had the highest quantity and quality, and was used for extraction from 84 tumor tissues. Nucleic acids were successfully extracted from all biopsies, and the amount of tumor (in millimeter) was found to have the strongest association with quantity and quality of nucleic acids.CONCLUSIONS: To conclude, this study shows that the choice of nucleic acid extraction kit affects the quantity and quality of extracted products. Furthermore, we show that extraction of nucleic acids from archival formalin-fixed prostate biopsies is possible, allowing molecular studies to be performed on this valuable sample collection.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Archival formalin-fixed paraffin-embedded tissue
- Nucleic acid isolation kits
- Prostate biopsies
- RNA integrity number
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- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Carlsson, Jessic ...
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Davidsson, Sabin ...
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Fridfeldt, Jonna
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Giunchi, Frances ...
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Fiano, Valentina
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Grasso, Chiara
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visa fler...
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Zelic, Renata
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Richiardi, Loren ...
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Andrén, Ove, 196 ...
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Pettersson, Andr ...
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Fiorentino, Mich ...
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Akre, Olof
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Urologi och njur ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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BMC Medical Rese ...
- Av lärosätet
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Örebro universitet
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Karolinska Institutet