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Platelet function t...
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Boknäs, Niklas,1979-Linköpings universitet,Avdelningen för klinisk kemi,Medicinska fakulteten,Region Östergötland, Hematologiska kliniken US,Australian Centre for Blood Diseases, Monash University, Melbourne, Australia
(författare)
Platelet function testing at low platelet counts : When can you trust your analysis?
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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Wiley-Blackwell,2019
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:oru-73960
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-73960URI
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https://doi.org/10.1002/rth2.12193DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-164457URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Funding Agencies:Linköpings UniversitetRegion ÖstergötlandLiU Fund of U; LiU Research Fellows Programme
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Funding Agencies|Linkopings Universitet; Hjart-Lungfonden [2017-0318]; Region Ostergotland; Swedish Medical Society [SLS-787211]; LiU Fund of U; LiU Research Fellows Programme
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Background: Although flow cytometry is often brought forward as a preferable method in the setting of thrombocytopenia, the relative effects of low sample counts on results from flow cytometry-based platelet function testing (FC-PFT) in comparison with light transmission aggregometry (LTA) and multiple electrode aggregometry (MEA) has not been reported.Objectives: To compare the effects of different sample platelet counts (10, 50, 100, and 200x10(9)L(-1)) on platelet activation measured with FC-PFT, LTA, and MEA using the same anticoagulant and agonist concentrations as for the commercial MEA test.Methods: Platelets were stimulated with two commonly used platelet agonists (ADP [6.5 mu molL(-1)] and PAR1-AP [TRAP, 32 mu molL(-1)]). The specified sample platelet counts were obtained by combining platelet-rich and platelet poor hirudinized plasma in different proportions with or without red blood cells.Results: For FC, P-selectin exposure and PAC-1 binding was reduced at 10x10(9)L(-1) after stimulation with PAR1-AP (by approximately 20% and 50%, respectively), but remained relatively unchanged when ADP was used as agonist (n=9). The platelet count-dependent effects observed with PAR1-AP were eliminated when samples were pre-incubated with apyrase, implying that reduced purinergic signaling was the main underlying factor (n=5). Both aggregometry-based PFTs showed a 50% reduction at 50x10(9)L(-1) and more than 80% reduction at 10x10(9)L(-1), irrespective of agonist used (n=7).Conclusions: Although FC-PFT is generally preferable to aggregometry-based PFTs in situations with low sample platelet counts, a careful optimization of experimental parameters is still required in order to eliminate platelet count-related effects.
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Macwan, AnkitLinköpings universitet,Avdelningen för klinisk kemi,Medicinska fakulteten(Swepub:liu)ankma14
(författare)
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Södergren, Anna L.,1985-Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)annso15
(författare)
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Ramström, Sofia,1973-Linköpings universitet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Cardiovascular Research Centre, Örebro University, School of Medical Sciences, Örebro, Sweden,Avdelningen för klinisk kemi,Medicinska fakulteten,Region Östergötland, Klinisk kemi(Swepub:liu)sofra86
(författare)
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Linköpings universitetAvdelningen för klinisk kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Research and Practice in Thrombosis and Haemostasis: Wiley-Blackwell3:2, s. 285-2902475-0379
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