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Performance of PCR/...
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Strålin, KristofferKarolinska Institutet
(författare)
Performance of PCR/electrospray ionization-mass spectrometry on whole blood for detection of bloodstream microorganisms in patients with suspected sepsis
- Artikel/kapitelEngelska2020
Förlag, utgivningsår, omfång ...
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American Society for Microbiology,2020
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:oru-84767
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-84767URI
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https://doi.org/10.1128/JCM.01860-19DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:144821107URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding Agencies:Ibis Biosciences, a division of Abbott Abbott LaboratoriesCepheid ContraFect Nabriva Therapeutics AG Lajolla Pharmaceuticals Alere Corporation Spectral Diagnostics Ferring Pharmaceuticals Inflammix VICTASm Sanofi Pasteur Inc. Curetis GmbH PfizerMerck & CompanyCidara Therapeutics Inc. Shire Aridis Pharmaceuticals Inc. Epigenomics Inc. Roche Holding GenentechFinch Therapeutics Seres Therapeutics Inc. Diasorin Molecular Janssen Research and Development NeuMoDx Molecular Iterum Therapeutics International
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Blood culture (BC) often fails to detect bloodstream microorganisms in sepsis. However, molecular diagnostics hold great potential. The molecular method PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) can detect DNA from hundreds of different microorganisms in whole blood. The aim of the present study was to evaluate the performance of this method in a multicenter study including 16 teaching hospitals in the USA (n=13) and Europe (n=3). First, on 2,754 contrived whole blood samples, with or without spiked microorganisms, PCR/ESI-MS produced 99.1% true positive and 97.2% true negative results. Secondly, among 1,460 patients with suspected sepsis (sepsis-2 definition), BC and PCR/ESI-MS on whole blood were positive in 14.6% and 25.6% of cases, respectively, with the following result combinations: BC+/PCR/ESI-MS-, 4.3%; BC+/PCR/ESI-MS+, 10.3%; BC-/PCR/ESI-MS+, 15.3%; and BC-/PCR/ESI-MS-, 70.1%. Compared with BC, PCR/ESI-MS showed the following sensitivities (coagulase-negative staphylococci not included): Gram-positive bacteria, 58%; Gram-negative bacteria, 78%; and Candida species, 83%. The specificities were > 94% for all individual species. Patients treated with prior antimicrobial medications (n=603) had significantly increased PCR/ESI-MS positivity rates compared with patients without prior antimicrobial treatment, 31% vs 22% (p<0.0001), with pronounced differences for Gram-negative bacteria and Candida species. In conclusion, PCR/ESI-MS showed excellent performance on contrived samples. On clinical samples, it showed high specificities, moderately high sensitivities for Gram-negative bacteria and Candida species, and elevated positivity rates during antimicrobial treatment. These promising results encourage further development of molecular diagnostics on whole blood for detection of bloodstream microorganisms in sepsis.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Rothman, Richard E.Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
(författare)
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Özenci, VolkanKarolinska Institutet
(författare)
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Barkataki, KieronDepartment of Emergency Medicine, Kern Medical Center, Bakersfield, California, USA
(författare)
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Brealey, DavidDivision of Critical Care, NIHR Biomedical Research Centre at University of College London Hospitals NHS Foundation Trust, London, United Kingdom
(författare)
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Dhiman, NeelamMolecular Infectious Diseases, med fusion/Quest Diagnostics, Lewisville, Texas, USA
(författare)
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Poling, LaraAthoGen Testing, Carlsbad, California, USA
(författare)
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Kurz, Michael C.Department of Emergency Medicine, University of Alabama School of Medicine, Birmingham, Alabama, USA
(författare)
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Limaye, Ajit P.University of Washington, Seattle, Washington, USA
(författare)
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LoVecchio, FrankUniversity of Arizona, Maricopa Medical Center, Phoenix, Arizona, USA
(författare)
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Lowery, KristinAthoGen Testing, Carlsbad, California, USA
(författare)
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Miller, Loren G.Lundquist Institute at Harbor-UCLA Medical Center, Torrance, California, USA
(författare)
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Moran, Gregory J.Department of Emergency Medicine, Olive View-UCLA Medical Center, Sylmar, California, USA
(författare)
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Overcash, J. ScotteStudySite, Sharp Chula Vista, San Diego, California, USA
(författare)
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Parekh, AmishaDepartment of Emergency Medicine, New York Methodist Hospital, New York, New York, USA
(författare)
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Peacock, W. FrankBaylor College of Medicine, Houston, Texas, USA
(författare)
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Rivers, Emanuel P.Department of Emergency Medicine and Surgery, Henry Ford Hospital, Detroit, Michigan, USA; Department of Surgery, Henry Ford Hospital, Detroit, Michigan, USA
(författare)
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Sims, MatthewBeaumont Health, Royal Oak and Oakland University William Beaumont School of Medicine, Rochester, Michigan, USA
(författare)
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Stubbs, Amy M.Department of Emergency Medicine, Truman Medical Center, University of Missouri-Kansas City, Kansas City, Missouri, USA
(författare)
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Sundqvist, Martin,1974-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Laboratory Medicine, Clinical Microbiology(Swepub:oru)mist
(författare)
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Ullberg, MånsKarolinska Institutet
(författare)
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Carroll, Karen C.Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
(författare)
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Karolinska InstitutetDepartment of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Clinical Microbiology: American Society for Microbiology58:9, s. e01860-190095-11371098-660X
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Strålin, Kristof ...
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Rothman, Richard ...
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Özenci, Volkan
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Barkataki, Kiero ...
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Brealey, David
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Dhiman, Neelam
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visa fler...
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Poling, Lara
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Kurz, Michael C.
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Limaye, Ajit P.
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LoVecchio, Frank
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Lowery, Kristin
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Miller, Loren G.
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Moran, Gregory J ...
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Overcash, J. Sco ...
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Parekh, Amisha
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Peacock, W. Fran ...
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Rivers, Emanuel ...
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Sims, Matthew
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Stubbs, Amy M.
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Sundqvist, Marti ...
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Ullberg, Måns
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Carroll, Karen C ...
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