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Rat perichondrium t...
Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage
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- Dou, Zelong (författare)
- Karolinska Institutet
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- Muder, Daniel (författare)
- Uppsala universitet,Ortopedi,Falu Lasarett, Dept Orthoped, Lasarettsvagen 10, SE-79182 Falun, Sweden.
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- Baroncelli, Marta (författare)
- Karolinska Institutet
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- Bendre, Ameya (författare)
- Karolinska Institutet
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- Gkourogianni, Alexandra (författare)
- Karolinska Institutet
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- Ottosson, Lars (författare)
- Karolinska Institutet
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- Vedung, Torbjörn (författare)
- Uppsala universitet,Ortopedi,Elisabeth Hosp, Aleris Hlthcare, Uppsala, Sweden.
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- Nilsson, Ola, 1970- (författare)
- Karolinska Institutet,Örebro universitet,Institutionen för medicinska vetenskaper,Division of Pediatric Endocrinology and Center for Molecular Medicine, L8:01, Department of Women's and Children's Health, Karolinska Institutet and University Hospital, Stockholm, Sweden; School of Medical Sciences, Örebro University Hospital, Örebro, Sweden,Karolinska Inst, Div Pediat Endocrinol, Stockholm, Sweden.;Karolinska Inst & Univ Hosp, Ctr Mol Med L8 01, Dept Womens & Childrens Hlth, SE-17176 Stockholm, Sweden.;Univ Hosp, Stockholm, Sweden.;Örebro Univ & Univ Hosp, Sch Med Sci, Örebro, Sweden.
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(creator_code:org_t)
- Elsevier, 2021
- 2021
- Engelska.
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Ingår i: Bone. - : Elsevier. - 8756-3282 .- 1873-2763. ; 151
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- OBJECTIVE: Perichondrium autotransplants have been used to reconstruct articular surfaces destroyed by infection or trauma. However, the role of the transplanted perichondrium in the healing of resurfaced joints have not been investigated.DESIGN: Perichondrial and periosteal tissues were harvested from rats hemizygous for a ubiquitously expressed enhanced green fluorescent protein (EGFP) transgene and transplanted into full-thickness articular cartilage defects at the trochlear groove of distal femur in wild-type littermates. As an additional control, cartilage defects were left without a transplant (no transplant control). Distal femurs were collected 3, 14, 56, 112 days after surgery.RESULTS: Tracing of transplanted cells showed that both perichondrium and periosteum transplant-derived cells made up the large majority of the cells in the regenerated joint surfaces. Perichondrium transplants contained SOX9 positive cells and with time differentiated into a hyaline cartilage that expanded and filled out the defects with Col2a1-positive and Col1a1-negative chondrocytes and a matrix rich in proteoglycans. At later timepoints the cartilaginous perichondrium transplants were actively remodeled into bone at the transplant-bone interface and at post-surgery day 112 EGFP-positive perichondrium cells at the articular surface were positive for Prg4. Periosteum transplants initially lacked SOX9 expression and despite a transient increase in SOX9 expression and chondrogenic differentiation, remained Col1a1 positive, and were continuously thinning as periosteum-derived cells were incorporated into the subchondral compartment.CONCLUSIONS: Perichondrium and periosteum transplanted to articular cartilage defects did not just stimulate regeneration but were themselves transformed into cartilaginous articular surfaces. Perichondrium transplants developed into an articular-like, hyaline cartilage, whereas periosteum transplants appeared to produce a less resilient fibro-cartilage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Articular cartilage
- Chondrocyte differentiation
- Injury healing
- Perichondrium
- Transplantation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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