Characterisation of Postia placenta colonisation during 36 weeks in acetylated southern yellow pine sapwood at three acetylation levels including genomic DNA and gene expression quantification of the fungus

• One way to protect timber in service against basidiomycete deterioration is by means of acetylation via reaction with acetic anhydride. The reason why acetylated wood (WAc) is resistant against decay fungi is still not exactly understood. The aim of this study was to contribute to this field of science, and Postia placenta colonisation after 4, 12, 20, 28 and 36 weeks was observed at three acetylation levels of Pinus spp. sapwood. Mass loss (ML) and wood moisture content (MC) data reflected the acetylation levels. The initial equilibrium MC (EMC) proved to be a good indicator of subsequent ML. Genomic DNA quantification showed P. placenta colonisation in all samples, also in samples where no ML were detectable. The number of expressed gene transcripts was limited, but the findings supported the results of previous studies: WAc seems to have some resistance against oxidative mechanisms, which are part of the metabolism of P. placenta. This leads to a delay in decay initiation, a delay in expression of genes involved in enzymatic depolymerisation, and a slower decay rate. The magnitudes of these effects are presented for each acetylation level. The data also imply that there is no absolute decay threshold at high acetylation levels, but instead a significant delay of decay initiation and a slower decay rate.

Ämnesord

LANTBRUKSVETENSKAPER  -- Lantbruksvetenskap, skogsbruk och fiske (hsv//swe)

AGRICULTURAL SCIENCES  -- Agriculture, Forestry and Fisheries (hsv//eng)

Nyckelord

acetylation

carbohydrate metabolism

equilibrium moisture content

gene expression

genomic DNA (gDNA)

mass loss

oxidative metabolism

quantitative real-time polymerase chain reaction (PCR)

southern yellow pine

weight percent gain

Decay (organic)

DNA

Fungi

Genes

Metabolism

Moisture

Moisture determination

Physiology

Polymerase chain reaction

Wood

Wood products

Equilibrium moisture contents

Genomic DNA

Quantitative real-time polymerase chain reaction

Southern yellow pines

Weight percent gain

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)