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Using fluorescent p...
Using fluorescent probes and FRAP to investigate macromolecule diffusion in steam-exploded wood
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- Kvist, Patric (författare)
- KTH,Wallenberg Wood Science Center,Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;Chalmers Univ Technol,;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden.,Chalmers tekniska högskola,Chalmers University of Technology
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- Schuster, Erich (författare)
- RISE,Jordbruk och livsmedel,Chalmers University of Technology, Sweden,RISE Agrifood & Biosci, Prod Design & Percept, Box 5401, S-40229 Gothenburg, Sweden.;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden.,RISE Research Institutes of Sweden
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- Loren, Niklas (författare)
- RISE,Jordbruk och livsmedel,Chalmers University of Technology, Sweden,Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;RISE Agrifood & Biosci, Prod Design & Percept, Box 5401, S-40229 Gothenburg, Sweden.;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden.,Chalmers tekniska högskola
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- Rasmuson, Anders (författare)
- KTH,Wallenberg Wood Science Center,Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;Chalmers Univ Technol,,Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2018-07-25
- 2018
- Engelska.
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Ingår i: Wood Science and Technology. - : Springer Science and Business Media LLC. - 0043-7719 .- 1432-5225. ; 52:5, s. 1395-1410
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Abstract
Ämnesord
Stäng
- Diffusion of fluorescently labeled dextran of varying molecular weight in wood pretreated by steam explosion was studied with a confocal microscope. The steam explosion experiments were conducted at relatively mild conditions relevant for materials biorefinery at a pressure of 14 bars for 10 min. The method of fluorescence recovery after photobleaching (FRAP) was used to perform diffusion measurements locally in the wood microstructure. It was found that the FRAP methodology can be used to observe differences in the diffusion coefficient based on localization in the microstructure, i.e., earlywood, latewood, and cell wall. Microscopic changes due to steam explosion were seen to increase diffusion of the smaller 3-kDa dextran diffusion probe in the earlywood, while the latewood structure was not affected in any significant way. Macroscopic changes to the structure in the form of ruptures due to the steam explosion pretreatment were observed to increase the rate of diffusion for the larger 40-kDa dextran probe.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Materialteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Kompositmaterial och -teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Composite Science and Engineering (hsv//eng)
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
Nyckelord
- Dextran
- Explosions
- Fluorescence
- Microstructure
- Photobleaching
- Probes
- Steam
- Steam engineering
- Biorefineries
- Cell walls
- Diffusion measurements
- Earlywood
- Fluorescence recovery after photobleaching
- Fluorescent probes
- Pre-Treatment
- Steam explosion
- Diffusion
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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