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  • Gerits, EvelienKatholieke Universiteit Leuven, Belgium (author)

Antibacterial activity of a new broad-spectrum antibiotic covalently bound to titanium surfaces

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • 2016-04-06
  • John Wiley and Sons Inc.2016
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:ri-43909
  • https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-43909URI
  • https://doi.org/10.1002/jor.23238DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Biofilm-associated infections, particularly those caused by Staphylococcus aureus, are a major cause of implant failure. Covalent coupling of broad-spectrum antimicrobials to implants is a promising approach to reduce the risk of infections. In this study, we developed titanium substrates on which the recently discovered antibacterial agent SPI031, a N-alkylated 3, 6-dihalogenocarbazol 1-(sec-butylamino)-3-(3,6-dichloro-9H-carbazol-9-yl)propan-2-ol, was covalently linked (SPI031-Ti). We found that SPI031-Ti substrates prevent biofilm formation of S. aureus and Pseudomonas aeruginosa in vitro, as quantified by plate counting and fluorescence microscopy. To test the effectiveness of SPI031-Ti substrates in vivo, we used an adapted in vivo biomaterial-associated infection model in mice in which SPI031-Ti substrates were implanted subcutaneously and subsequently inoculated with S. aureus. Using this model, we found a significant reduction in biofilm formation (up to 98%) on SPI031-Ti substrates compared to control substrates. Finally, we demonstrated that the functionalization of the titanium surfaces with SPI031 did not influence the adhesion and proliferation of human cells important for osseointegration and bone repair. In conclusion, these data demonstrate the clinical potential of SPI031 to be used as an antibacterial coating for implants, thereby reducing the incidence of implant-associated infections.

Subject headings and genre

  • biofilm
  • Pseudomonas aeruginosa
  • SPI031
  • Staphylococcus aureus
  • titanium
  • 3
  • 6 dihalogenocarbazol 1 ( sec butylamino) 3 ( 3
  • 6 dichloro 9h carbazol 9 yl)propan 2 ol
  • antibiotic agent
  • biomaterial
  • carbazole derivative
  • unclassified drug
  • antiinfective agent
  • animal experiment
  • antibacterial activity
  • Article
  • bacterial strain
  • bone implant
  • bone regeneration
  • bone remodeling
  • cell adhesion
  • cell proliferation
  • controlled study
  • covalent bond
  • drug delivery device
  • drug efficacy
  • experimental infection
  • fluorescence microscopy
  • human
  • human cell
  • in vitro study
  • in vivo study
  • mouse
  • nonhuman
  • plate count
  • priority journal
  • reduction
  • titanium cage
  • animal
  • Bagg albino mouse
  • drug effects
  • female
  • microbial sensitivity test
  • Prosthesis-Related Infections
  • Animals
  • Anti-Infective Agents
  • Carbazoles
  • Mice
  • Inbred BALB C
  • Microbial Sensitivity Tests

Added entries (persons, corporate bodies, meetings, titles ...)

  • Kucharíkova, SonaKatholieke Universiteit Leuven, Belgium (author)
  • Van Dijck, Patrick V.Katholieke Universiteit Leuven, Belgium; Hemoteq AG, Germany (author)
  • Erdtmann, MartinHemoteq AG, Germany (author)
  • Krona, AnnikaRISE,Structure Design(Swepub:ri)annikakr@ri.se (author)
  • Lövenklev, MariaRISE,Structure Design (author)
  • Fröhlich, MirjamEducell Ltd, Slovenia; University of Ljubljana, Slovenia (author)
  • Dovgan, BarbaraEducell Ltd, Slovenia (author)
  • Impellizzeri, FredericBiotech International, France (author)
  • Braem, AnnabelKatholieke Universiteit Leuven, Belgium (author)
  • Vleugels, JozefKatholieke Universiteit Leuven, Belgium (author)
  • Robijns, Stijn Chris ArnoldKatholieke Universiteit Leuven, Belgium (author)
  • Steenackers, Hans P.L.Katholieke Universiteit Leuven, Belgium (author)
  • Vanderleyden, Jos X.Katholieke Universiteit Leuven, Belgium (author)
  • De Brucker, KatrijnKatholieke Universiteit Leuven, Belgium (author)
  • Thevissen, KarinKatholieke Universiteit Leuven, Belgium (author)
  • Cammue, Bruno P.A.Katholieke Universiteit Leuven, Belgium; Vlaams Instituut voor Biotechnologie, Belgium (author)
  • Fauvart, MaartenIMEC, Belgium (author)
  • Verstraeten, NatalieKatholieke Universiteit Leuven, Belgium (author)
  • Michiels, JanKatholieke Universiteit Leuven, Belgium (author)
  • Katholieke Universiteit Leuven, BelgiumKatholieke Universiteit Leuven, Belgium; Hemoteq AG, Germany (creator_code:org_t)

Related titles

  • In:Journal of Orthopaedic Research: John Wiley and Sons Inc.34:12, s. 2191-21980736-02661554-527X

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