Microwave-assisted extraction of polysaccharides from the marshmallow roots : Optimization, purification, structure, and bioactivity

• The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied. The highest MAE-yield (14.47%) was optimized at 457.32 W and 75 °C for 26 min. The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da. The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively. The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose. The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL). MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells. The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions. 

Nyckelord

Althaea officinalis L.

Biological properties

Extraction

FTIR

Functional properties

Microwave

polysaccharide

Antioxidants

Fourier transform infrared spectroscopy

Gel permeation chromatography

Glucose

Ion exchange

Morphology

Optimization

Purification

Scanning electron microscopy

Surface morphology

Anti-proliferative activities

Anti-tumor activities

Antibacterial functions

Biological characterization

Concentration-dependent manners

Gel-filtration chromatography

Microwave-assisted extraction

Monosaccharide composition

Polysaccharides

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)