Efficient prevention of marine biofilm formation employing a surface-grafted repellent marine peptide

• Creation of surfaces resistant to the formation of microbial biofilms via biomimicry has been heralded as a promising strategy to protect a range of different materials ranging from boat hulls to medical devices and surgical instruments. In our current study, we describe the successful transfer of a highly effective natural marine biofilm inhibitor to the 2D surface format. A series of cyclic peptides inspired by the natural equinatoxin II protein produced by Beadlet anemone (Actinia equine) have been evaluated for their ability to inhibit the formation of a mixed marine microbial consortium on polyamide reverse osmosis membranes. In solution, the peptides are shown to effectively inhibit settlement and biofilm formation in a nontoxic manner down to 1 nM concentrations. In addition, our study also illustrates how the peptides can be applied to disperse already established biofilms. Attachment of a hydrophobic palmitic acid tail generates a peptide suited for strong noncovalent surface interactions and allows the generation of stable noncovalent coatings. These adsorbed peptides remain attached to the surface at significant shear stress and also remain active, effectively preventing the biofilm formation over 24 h. Finally, the covalent attachment of the peptides to an acrylate surface was also evaluated and the prepared coatings display a remarkable ability to prevent surface colonization at surface loadings of 55 ng/cm2 over 48 h. The ability to retain the nontoxic antibiofilm activity, documented in solution, in the covalent 2D-format is unprecedented, and this natural peptide motif displays high potential in several material application areas.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Materials (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Biomaterial (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Bio Materials (hsv//eng)

Nyckelord

Antifouling

Marine biofilm

Nontoxic

Peptide

Reverse osmosis

Surface grafting

Biofilms

Biomimetics

Boat instruments

Coatings

Osmosis membranes

Palmitic acid

Shear stress

Surgical equipment

Biofilm formation

Covalent attachment

Material application

Microbial biofilm

Microbial consortia

Noncovalent surfaces

Surface colonization

Surgical instrument

Peptides

marine biofilm

antifouling

peptide

nontoxic

surface grafting

reverse osmosis

reverse-osmosis membranes

antimicrobial peptides

compounds

synthetic analogs

natural-products

equinatoxin-ii

attachment

immobilization

adsorption

albumin

Science & Technology - Other Topics

Materials Science

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)