Anti-colonization effect of au surfaces with self-assembled molecular monolayers functionalized with antimicrobial peptides on s. Epidermidis

• Medical devices with an effective anti-colonization surface are important tools for com-batting healthcare-associated infections. Here, we investigated the anti-colonization efficacy of antimicrobial peptides covalently attached to a gold model surface. The gold surface was modified by a self-assembled polyethylene glycol monolayer with an acetylene terminus. The peptides were covalently connected to the surface through a copper-catalyzed [3 + 2] azide-acetylene coupling (CuAAC). The anti-colonization efficacy of the surfaces varied as a function of the antimicrobial activity of the peptides, and very effective surfaces could be prepared with a 6 log unit reduction in bacterial colonization. © 2021 by the authors. 

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomaterials Science (hsv//eng)

Nyckelord

Anti-colonization

Antifouling

Antimicrobial peptide

Antimicrobial surface

Certika

Self-assembled monolayer

ToF-SIMS imaging

acetylene

copper

dichloromethane

gold

macrogol

macrogol 200

macrogol 400

phenylalanine

polypeptide antibiotic agent

self assembled monolayer

tryptophan

antibiotic sensitivity

antimicrobial activity

Article

bacterial colonization

colony forming unit

column chromatography

drug synthesis

electrospray mass spectrometry

Escherichia coli

Gram negative bacterium

healthcare associated infection

high performance liquid chromatography

lipophilicity

minimum inhibitory concentration

nonhuman

proton nuclear magnetic resonance

Staphylococcus epidermidis

time of flight mass spectrometry

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)