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A Lab-in-a-Fiber op...
A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection
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- Parker, Helen E. (författare)
- KTH,Laserfysik,Heriot Watt Univ, Scottish Univ Phys Alliance SUPA, Inst Photon & Quantum Sci, Edinburgh EH14 4AS, Midlothian, Scotland.
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- Sengupta, Sanghamitra (författare)
- KTH,Laserfysik,AMOLF, Sci Pk 104, NL-1098 XG Amsterdam, Netherlands.
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- Harish, Achar Vasant (författare)
- KTH,Laserfysik
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- Soares, Ruben R. G. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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- Jönsson, Håkan, PhD, 1979- (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Margulis, Walter (författare)
- KTH,RISE,KTH Royal Institute of Technology, Sweden,Laserfysik,Res Inst Sweden RISE, S-16419 Stockholm, Sweden.
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Karolinska Inst, AIMES Ctr Adv Integrated Med & Engn Sci, Stockholm, Sweden.;KTH Royal Inst Technol, Stockholm, Sweden.
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- Laurell, Fredrik, professor, 1957- (författare)
- KTH,Laserfysik
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(creator_code:org_t)
- 2022-03-03
- 2022
- Engelska.
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Ingår i: Scientific Reports. - : Nature Research. - 2045-2322. ; 12:1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Microfluidics has emerged rapidly over the past 20 years and has been investigated for a variety of applications from life sciences to environmental monitoring. Although continuous-flow microfluidics is ubiquitous, segmented-flow or droplet microfluidics offers several attractive features. Droplets can be independently manipulated and analyzed with very high throughput. Typically, microfluidics is carried out within planar networks of microchannels, namely, microfluidic chips. We propose that fibers offer an interesting alternative format with key advantages for enhanced optical coupling. Herein, we demonstrate the generation of monodisperse droplets within a uniaxial optofluidic Lab-in-a-Fiber scheme. We combine droplet microfluidics with laser-induced fluorescence (LIF) detection achieved through the development of an optical side-coupling fiber, which we term a periscope fiber. This arrangement provides stable and compact alignment. Laser-induced fluorescence offers high sensitivity and low detection limits with a rapid response time making it an attractive detection method for in situ real-time measurements. We use the well-established fluorophore, fluorescein, to characterize the Lab-in-a-Fiber device and determine the generation of ∼ 0.9 nL droplets. We present characterization data of a range of fluorescein concentrations, establishing a limit of detection (LOD) of 10 nM fluorescein. Finally, we show that the device operates within a realistic and relevant fluorescence regime by detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products in the context of COVID-19 diagnostics. The device represents a step towards the development of a point-of-care droplet digital RT-LAMP platform. © 2022, The Author(s).
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Nyckelord
- fluorescence
- isolation and purification
- lab on a chip
- laser
- virus
- Lab-On-A-Chip Devices
- Lasers
- Viruses
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Parker, Helen E.
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Sengupta, Sangha ...
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Harish, Achar Va ...
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Soares, Ruben R. ...
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Jönsson, Håkan, ...
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Margulis, Walter
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Russom, Aman, Pr ...
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Laurell, Fredrik ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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och Atom och molekyl ...
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Scientific Repor ...
- Av lärosätet
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RISE
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Kungliga Tekniska Högskolan
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Karolinska Institutet