Personalized tissue-engineered arteries as vascular graft transplants : A safety study in sheep

• Patients with cardiovascular disease often need replacement or bypass of a diseased blood vessel. With disadvantages of both autologous blood vessels and synthetic grafts, tissue engineering is emerging as a promising alternative of advanced therapy medicinal products for individualized blood vessels. By reconditioning of a decellularized blood vessel with the recipient's own peripheral blood, we have been able to prevent rejection without using immunosuppressants and prime grafts for efficient recellularization in vivo. Recently, decellularized veins reconditioned with autologous peripheral blood were shown to be safe and functional in a porcine in vivo study as a potential alternative for vein grafting. In this study, personalized tissue engineered arteries (P-TEA) were developed using the same methodology and evaluated for safety in a sheep in vivo model of carotid artery transplantation. Five personalized arteries were transplanted to carotid arteries and analyzed for safety and patency as well as with histology after four months in vivo. All grafts were fully patent without any occlusion or stenosis. The tissue was well cellularized with a continuous endothelial cell layer covering the luminal surface, revascularized adventitia with capillaries and no sign of rejection or infection. In summary, the results indicate that P-TEA is safe to use and has potential as clinical grafts. 

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Nyckelord

ATMP

Blood vessels

Recellularization

Regenerative medicine

Scaffold

Tissue engineering

amoxicillin

attane

buprenorphine

carprofen

clopidogrel

dalteparin

dexmedetomidine

heparin

isoflurane

norocarp

poliglecaprone

promea

propofol

receptor type tyrosine protein phosphatase C

vetergesic

vetrimoxin

adventitia

anatomical concepts

angiography

animal experiment

animal model

animal tissue

antibiotic prophylaxis

arterial stiffness

artery anastomosis

artery transplantation

Article

biomechanics

biopsy technique

blood flow

blood vessel graft

blood vessel parameters

blood vessel wall

burst pressure

capillary

carotid artery transplantation

confocal laser scanning microscopy

controlled study

decellularization

decellularized blood vessel

device safety

DNA extraction

end to end anastomosis

endothelial cell layer

evening dosage

extracellular matrix

failure strain

female

fluorescence microscopy

full luminal endothelialization

histology

hyperplasia

immunohistochemistry

intimal hyperplasia

luminal surface

medical procedures

morning dosage

nonhuman

reconditioning of vascular graft

revascularization

scanning electron microscopy

ATMP

Blood vessels

Recellularization

Regenerative medicine

Scaffold

Tissue engineering

amoxicillin

attane

buprenorphine

carprofen

clopidogrel

dalteparin

dexmedetomidine

heparin

isoflurane

norocarp

poliglecaprone

promea

propofol

receptor type tyrosine protein phosphatase C

vetergesic

vetrimoxin

adventitia

anatomical concepts

angiography

animal experiment

animal model

animal tissue

antibiotic prophylaxis

arterial stiffness

artery anastomosis

artery transplantation

Article

biomechanics

biopsy technique

blood flow

blood vessel graft

blood vessel parameters

blood vessel wall

burst pressure

capillary

carotid artery transplantation

confocal laser scanning microscopy

controlled study

decellularization

decellularized blood vessel

device safety

DNA extraction

end to end anastomosis

endothelial cell layer

evening dosage

extracellular matrix

failure strain

female

fluorescence microscopy

full luminal endothelialization

histology

hyperplasia

immunohistochemistry

intimal hyperplasia

luminal surface

medical procedures

morning dosage

nonhuman

reconditioning of vascular graft

revascularization

scanning electron microscopy

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)