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Deciphering the rol...
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Ranji, ParmidaUniversity of Gothenburg, Sweden
(författare)
Deciphering the role of FUS::DDIT3 expression and tumor microenvironment in myxoid liposarcoma development
- Artikel/kapitelEngelska2024
Förlag, utgivningsår, omfång ...
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BioMed Central Ltd,2024
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:ri-73289
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https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-73289URI
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https://doi.org/10.1186/s12967-024-05211-wDOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Open access funding provided by University of Gothenburg. This research was funded by Assar Gabrielssons Research Foundation; Johan Jansson Foundation for Cancer Research; Region Västra Götaland, Sweden; Swedish Cancer Society (2022-2080 and 2022-2214); Swedish Childhood Cancer Foundation (2022-0030); Swedish Research Council (2021-01008 and 2019-01273); the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965065 and 965580) and Sweden's Innovation Agency (2017-03737); the Sjöberg Foundation and Wilhelm and Martina Lundgren Foundation for Scientific Research.
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Background: Myxoid liposarcoma (MLS) displays a distinctive tumor microenvironment and is characterized by the FUS::DDIT3 fusion oncogene, however, the precise functional contributions of these two elements remain enigmatic in tumor development. Methods: To study the cell-free microenvironment in MLS, we developed an experimental model system based on decellularized patient-derived xenograft tumors. We characterized the cell-free scaffold using mass spectrometry. Subsequently, scaffolds were repopulated using sarcoma cells with or without FUS::DDIT3 expression that were analyzed with histology and RNA sequencing. Results: Characterization of cell-free MLS scaffolds revealed intact structure and a large variation of protein types remaining after decellularization. We demonstrated an optimal culture time of 3 weeks and showed that FUS::DDIT3 expression decreased cell proliferation and scaffold invasiveness. The cell-free MLS microenvironment and FUS::DDIT3 expression both induced biological processes related to cell-to-cell and cell-to-extracellular matrix interactions, as well as chromatin remodeling, immune response, and metabolism. Data indicated that FUS::DDIT3 expression more than the microenvironment determined the pre-adipocytic phenotype that is typical for MLS. Conclusions: Our experimental approach opens new means to study the tumor microenvironment in detail and our findings suggest that FUS::DDIT3-expressing tumor cells can create their own extracellular niche.
Ämnesord och genrebeteckningar
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MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
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MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
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MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
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MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
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Animals; Cell Line
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Tumor; Cell Proliferation; Cell-Free System; Extracellular Matrix; Gene Expression Regulation
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Neoplastic; Humans; Liposarcoma
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Myxoid; Mice; Oncogene Proteins
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Fusion; RNA-Binding Protein FUS; Tissue Scaffolds; Tumor Microenvironment; eosin; growth arrest and DNA damage inducible protein 153; hematoxylin; HLA antigen; major histocompatibility antigen class 2; RNA binding protein FUS; FUS protein
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human; FUS-DDIT3 fusion protein
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human; oncogene fusion protein; RNA binding protein FUS; adipogenesis; animal cell; animal experiment; animal model; antigen presentation; Article; cell adhesion; cell culture; cell cycle; cell growth; cell infiltration; cell interaction; cell invasion; cell migration; cell proliferation; chromatin assembly and disassembly; controlled study; decellularization; down regulation; endocytosis; experimental model; extracellular matrix; female; functional enrichment analysis; gene expression; gene expression profiling; gene fusion; genetic transcription; glycolysis; histology; HT-1080 cell line; human; human cell; immune response; in vivo study; liquid chromatography-mass spectrometry; mass spectrometry; metabolism; mouse; myxosarcoma; nonhuman; nucleotide metabolism; oncogene; phenotype; protein expression; proteomics; RNA extraction; RNA sequencing; RNA synthesis; sarcoma cell; single cell RNA seq; tumor cell; tumor microenvironment; tumor xenograft; upregulation; animal; cell free system; chemistry; gene expression regulation; genetics; pathology; tumor cell line
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Jonasson, EmmaUniversity of Gothenburg, Sweden
(författare)
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Andersson, LisaUniversity of Gothenburg, Sweden
(författare)
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Filges, StefanUniversity of Gothenburg, Sweden
(författare)
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Luna Santamaría, ManuelUniversity of Gothenburg, Sweden
(författare)
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Vannas, ChristofferUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(författare)
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Dolatabadi, SoheilaUniversity of Gothenburg, Sweden
(författare)
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Gustafsson, AnnaUniversity of Gothenburg, Sweden
(författare)
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Myklebost, OlaOslo University Hospital, Norway; University of Bergen, Norway
(författare)
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Håkansson, JoakimRISE,Metodik för produktframtagning,University of Gothenburg, Sweden(Swepub:ri)JoakimHa@ri.se
(författare)
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Fagman, HenrikUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(författare)
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Landberg, GöranUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(författare)
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Åman, PierreUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(författare)
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Ståhlberg, AndersUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(författare)
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University of Gothenburg, SwedenUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Translational Medicine: BioMed Central Ltd221479-5876
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Till lärosätets databas
- Av författaren/redakt...
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Ranji, Parmida
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Jonasson, Emma
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Andersson, Lisa
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Filges, Stefan
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Luna Santamaría, ...
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Vannas, Christof ...
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visa fler...
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Dolatabadi, Sohe ...
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Gustafsson, Anna
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Myklebost, Ola
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Håkansson, Joaki ...
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Fagman, Henrik
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Landberg, Göran
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Åman, Pierre
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Ståhlberg, Ander ...
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