Fission yeast Iec1-Ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism

Ino80 is an ATP-dependent nucleosome-remodeling enzyme involved in transcription, replication, and the DNA damage response. Here, we characterize the fission yeast Ino80 and find that it is essential for cell viability. We show that the Ino80 complex from fission yeast mediates ATP-dependent nucleosome remodeling in vitro. The purification of the Ino80-associated complex identified a highly conserved complex and the presence of a novel zinc finger protein with similarities to the mammalian transcriptional regulator Yin Yang 1 (YY1) and other members of the GLI-Krüppel family of proteins. Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. We show that Iec1 is important for the correct expression of genes involved in nucleotide metabolism, including the ribonucleotide reductase subunit cdc22 and phosphate- and adenineresponsive genes. We find that Ino80 is recruited to a large number of promoter regions on phosphate starvation, including those of phosphate- and adenine-responsive genes that depend on Iec1 for correct expression. Iec1 is required for the binding of Ino80 to target genes and subsequent histone loss at the promoter and throughout the body of these genes on phosphate starvation. This suggests that the Iec1-Ino80 complex promotes transcription through nucleosome eviction.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
adenine
nucleotide
phosphate
protein kinase
protein subunit
ribonucleotide reductase
transcription factor YY1
zinc finger protein
article
cell viability
DNA damage
DNA repair
DNA replication
gene targeting
nonhuman
nucleosome
nucleotide metabolism
phosphate metabolism
priority journal
promoter region
protein binding
protein purification
transcription regulation
yeast
Amino Acid Sequence
Cell Cycle Proteins
Gene Expression Regulation
Fungal
Microarray Analysis
Molecular Sequence Data
Nucleosomes
Nucleotides
Phosphates
Schizosaccharomyces
Schizosaccharomyces pombe Proteins
Transcription Factors
Zinc Fingers
Mammalia
Schizosaccharomycetaceae

Aligianni, S. (author)
Durand-Dubief, M. (author)
Persson, J.Karolinska Institutet (author)
Will, W. R. (author)
Webster, J. (author)
Wheeler, L. (author)
Mathews, C. K. (author)
Elderkin, S. (author)
Oxley, D. (author)
Ekwall, KarlSödertörns högskola,Molekylärbiologi,Karolinska Institutet(Swepub:sh)SHKLEL (author)
Varga-Weisz, P. D. (author)
Karolinska InstitutetMolekylärbiologi (creator_code:org_t)

By the author/editor: Hogan, C. J.; Aligianni, S.; Durand-Dubief, M ...; Persson, J.; Will, W. R.; Webster, J.; show more...; Wheeler, L.; Mathews, C. K.; Elderkin, S.; Oxley, D.; Ekwall, Karl; Varga-Weisz, P. ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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