Fission yeast Iec1-Ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism

• Ino80 is an ATP-dependent nucleosome-remodeling enzyme involved in transcription, replication, and the DNA damage response. Here, we characterize the fission yeast Ino80 and find that it is essential for cell viability. We show that the Ino80 complex from fission yeast mediates ATP-dependent nucleosome remodeling in vitro. The purification of the Ino80-associated complex identified a highly conserved complex and the presence of a novel zinc finger protein with similarities to the mammalian transcriptional regulator Yin Yang 1 (YY1) and other members of the GLI-Krüppel family of proteins. Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. We show that Iec1 is important for the correct expression of genes involved in nucleotide metabolism, including the ribonucleotide reductase subunit cdc22 and phosphate- and adenineresponsive genes. We find that Ino80 is recruited to a large number of promoter regions on phosphate starvation, including those of phosphate- and adenine-responsive genes that depend on Iec1 for correct expression. Iec1 is required for the binding of Ino80 to target genes and subsequent histone loss at the promoter and throughout the body of these genes on phosphate starvation. This suggests that the Iec1-Ino80 complex promotes transcription through nucleosome eviction.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

adenine

nucleotide

phosphate

protein kinase

protein subunit

ribonucleotide reductase

transcription factor YY1

zinc finger protein

article

cell viability

DNA damage

DNA repair

DNA replication

gene targeting

nonhuman

nucleosome

nucleotide metabolism

phosphate metabolism

priority journal

promoter region

protein binding

protein purification

transcription regulation

yeast

Amino Acid Sequence

Cell Cycle Proteins

Gene Expression Regulation

Fungal

Microarray Analysis

Molecular Sequence Data

Nucleosomes

Nucleotides

Phosphates

Schizosaccharomyces

Schizosaccharomyces pombe Proteins

Transcription Factors

Zinc Fingers

Mammalia

Schizosaccharomycetaceae

Publication and Content Type

ref (subject category)

art (subject category)