DNA damage caused by etoposide and γ-irradiation induces gene conversion of the MHC in a mouse non-germline testis cell line

• We have explored the effects of γ-irradiation and etoposide on the gene conversion frequency between the endogenous major histocompatibility complex class II genes Abk and Ebd in a mouse testis cell line of non-germline origin with a polymerase chain reaction assay. Both γ-rays and etoposide were shown to increase the gene conversion frequency with up to 15-fold compared to untreated cells. Etoposide, which is an agent that stabilise a cleavable complex between DNA and DNA topoisomerase II, shows an increased induction of gene conversion events with increased dose of etoposide. Cells treated with γ-rays, which induce strand breaks, had an increased gene conversion frequency when they were subjected to low doses of irradiation, but increasing doses of irradiation did not lead to an increase of gene conversion events, which might reflect differences in the repair process depending on the extent and nature of the DNA damage. These results where DNA damage was shown to be able to induce gene conversion of endogenous genes in mouse testis cells suggests that the DNA repair system could be involved in the molecular genetic mechanism that results in gene conversion in higher eukaryotes like mammals.

Ämnesord

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Nyckelord

γ-Irradiation

DNA damage

Etoposide

Gene conversion

MHC

Mouse

carcinogen

DNA topoisomerase (ATP hydrolysing)

major histocompatibility antigen class 2

animal cell

article

controlled study

DNA repair

eukaryote

gamma irradiation

germ cell

immunogenetics

male

mammal

nonhuman

polymerase chain reaction

priority journal

testis cell

Amino Acid Sequence

Animals

Base Sequence

Cells

Cultured

Crosses

Genetic

DNA Mutational Analysis

Dose-Response Relationship

Drug

Dose-Response Relationship

Radiation

Gamma Rays

Genes

MHC Class II

Mice

Mice

Inbred BALB C

Mice

Inbred C3H

Molecular Sequence Data

Sequence Alignment

Testis

Animalia

Eukaryota

Mammalia

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)