Thermal unfolding of the archaeal DNA and RNA binding protein Ssh10

Wu, Xiaoqiu Oppermann, Madalina Berndt, Kurt D Bergman, Tomas Jornvall, Hans Knapp, Stefan Oppermann, Udo Research Support, Non-U.S. Gov't United States Biochemical and biophysical research communications Biochem Biophys Res Commun. 2008 Sep 5;373(4):482-7. Epub 2008 Jun 20.
The reversible thermal unfolding of the archaeal histone-like protein Ssh10b from the extremophile Sulfolobus shibatae was studied using differential scanning calorimetry and circular dichroism spectroscopy. Analytical ultracentrifugation and gel filtration showed that Ssh10b is a stable dimer in the pH range 2.5-7.0. Thermal denaturation data fit into a two-state unfolding model, suggesting that the Ssh10 dimer unfolds as a single cooperative unit with a maximal melting temperature of 99.9 degrees C and an enthalpy change of 134 kcal/mol at pH 7.0. The heat capacity change upon unfolding determined from linear fits of the temperature dependence of DeltaH(cal) is 2.55 kcal/(mol K). The low specific heat capacity change of 13 cal/(mol K residue) leads to a considerable flattening of the protein stability curve (DeltaG (T)) and results in a maximal DeltaG of only 9.5 kcal/mol at 320 K and a DeltaG of only 6.0 kcal/mol at the optimal growth temperature of Sulfolobus.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
*Hot Temperature
Amino Acid Sequence
Archaeal Proteins/chemistry/*metabolism
Calorimetry
Differential Scanning
Circular Dichroism
DNA-Binding Proteins/chemistry/*metabolism
Histones/chemistry/*metabolism
Hydrogen-Ion Concentration
Molecular Sequence Data
Protein Denaturation
Protein Folding
RNA-Binding Proteins/chemistry/*metabolism
Sulfolobus/*metabolism
Thermodynamics
Other research area
Annat forskningsområde

Ingår i:Biochemical and Biophysical Research Communications - BBRC: Elsevier BV373:4, s. 482-4870006-291X1090-2104

Av författaren/redakt...: Wu, X; Oppermann, M; Berndt, Kurt D; Bergman, T; Jörnvall, H; Knapp, S; visa fler...; Oppermann, U; visa färre...

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