The Program Asno for Computer-Supported Collection of Noe Upper Distance Constraints as Input for Protein-Structure Determination

• A new program, ASNO ('ASsign NOes'), for computer-supported NOE cross-peak assignments is described. ASNO is used for structure refinement in several rounds of NOESY cross-peak assignments and 3D structure calculations, where the preliminary structures are used as a reference to resolve ambiguities in NOE assignments which are otherwise based on the chemical shifts available from the sequence-specific resonance assignments. The practical use of ASNO for proteins is illustrated with the structure determination of Dendrotoxin K from Dendroaspis polylepis polylepis.

Ämnesord

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)

Nyckelord

assignment of noe distance constraints

caliba

diana

glomsa

habas

nmr structure

nuclear-magnetic-resonance

protein structure determination

structure refinement

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