An alternate pattern for globoside oligosaccharide expression in Haemophilus influenzae lipopolysaccharide: Structural diversity in nontypeable strain 1124

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:sh-31883" > An alternate patter...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Yildirim, Håkan H.Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet (author)

An alternate pattern for globoside oligosaccharide expression in Haemophilus influenzae lipopolysaccharide : Structural diversity in nontypeable strain 1124

Article/chapterEnglish2005

Publisher, publication year, extent ...

2005-03-08
American Chemical Society (ACS),2005
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:sh-31883
https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-31883URI
https://doi.org/10.1021/bi047480hDOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:1958224URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Common structural motifs of Haemophilus influenzae lipopolysaccharide (LPS) are globotetraose [beta-D-GalpNAc-(1 -> 3)-alpha-D-Galp-(1 -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Glcp] and its truncated versions globoside [alpha-D-Galp-(1 -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Glcp] and lactose [beta-D-Galp-(1 -> 4)-beta-D-Glcp] linked to the tenninal heptose (HepIII) of the triheptosyl inner-core moiety L-alpha-D-Hepp-(1 -> 2)-[PEA -> 6]-L-alpha-D-Hepp-(1 -> 3)L-alpha-D-Hepp-(1 -> 5)-[PPEA -> 4]-alpha-Kdo-(2 -> 6)-lipid A. We report here structural studies of LPS from nontypeable H. influenzae strain 1124 expressing these motifs linked to both the proximal heptose (HepI) and HepIII at the same time. This novel finding was obtained by structural studies of LPS using NMR techniques and electrospray ionization mass spectrometry (ESI-MS) on O-deacylated LPS and core oligosaccharide material (OS) as well as ESI-MSn on permethylated dephosphorylated OS. The use of defined mutants allowed us to confirm structures unambiguously and understand better the biosynthesis of each of the globotetraose units. We found that lgtC is involved in the expression of beta-D-Galp-(1 -> 4)-beta-D-Galp in both extensions, whereas lic2A directs only the expression Of beta-D-Ga1p-(1 -> 4)-beta-D-Glcp when linked to HepIII. The LPS of NTHi strain 1124 contained sialylated glycoforms that were identified by CE-ESI-MS/MS. A common sialylated structure in H. influenzae LPS is sialyllactose linked to HepIII. This structure exists in strain 1124. However, results for the lpsA mutant indicate that sialyllactose extends from HepI as well, a molecular environment for sialyllactose in H. influenzae that has not been reported previously. In addition, the LPS was found to carry phosphoryleholine, O-linked glycine, and a third PEA group which was linked to O3 of HepIII.

Subject headings and genre

NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng

Added entries (persons, corporate bodies, meetings, titles ...)

Li, J JNational Research Council of Canada, Ottawa, Canada (author)
Richards, J CNational Research Council of Canada, Ottawa, Canada (author)
Hood, D WJohn Radcliffe Hospital, Oxford, UK (author)
Moxon, E RJohn Radcliffe Hospital, Oxford, UK (author)
Schweda, Elke K. H.Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet(Swepub:sh)SHEESA (author)
Södertörns högskolaInstitutionen för livsvetenskaper (creator_code:org_t)

Related titles

In:Biochemistry: American Chemical Society (ACS)44:13, s. 5207-52240006-29601520-4995

Internet link

Find in a library

Biochemistry (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Yildirim, Håkan ...; Li, J J; Richards, J C; Hood, D W; Moxon, E R; Schweda, Elke K. ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...

Articles in the publication: Biochemistry

By the university: Södertörn University; Karolinska Institutet

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se