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The Interdomain Reg...
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Brooks, Andrew J
(author)
The Interdomain Region of Dengue NS5 ProteinInteracts with NS3 and Host Proteins
- Article/chapterEnglish2002
Publisher, publication year, extent ...
Numbers
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LIBRIS-ID:oai:DiVA.org:sh-6066
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https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-6066URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:vet swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Although dengue virus genome replication occurs in the cytoplasm of infected cells, it has been shown that the NS5 protein (RNA-dependent RNA polymerase) is hyperphosphorylated at a late stage in infection and localized to the cell nucleus. A 37 amino acid sequence of NS5 (residues 369-405) was shown to contain a functional nuclear localization signal (NLS) that interacted with the cellular nuclear transport factor, importin α/β heterodimer. Further studies using the yeast two-hybrid system revealed that the NS5 region (residues 320-368) immediately adjacent to the NLS contained an importin β-binding site that abuts or overlaps the binding site for the NS3 protein (protease/helicase). The importin β-binding site has also been shown to be a functional NLS (bNLS). Intriguingly, when both bNLS and NLS (residues 320-405) were present, the fused β -galactosidase protein did not accumulate in the nucleus. Here we provide a review of our studies on the NS5 interdomain region and compare it to other members of the Flavivirus genus in order to highlight the importance of this region as a possible target for developing broad-acting antiviral agent against dengue and other mechanistically-related viruses.
Subject headings and genre
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NS5 protein
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protein interactions
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NS3 protein
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nuclear localization signal
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Johansson, MagnusSödertörns högskola,Avdelning Naturvetenskap(Swepub:sh)shmsjn02
(author)
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Criswell, Erin
(author)
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Jans, David A
(author)
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Vasudevan, Subhash G
(author)
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Södertörns högskolaAvdelning Naturvetenskap
(creator_code:org_t)
Related titles
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In:Dengue Bulletin26, s. 155-1611020-895X
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