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Release of prostagl...
Release of prostaglandin D2 and leukotriene C4 in response to hyperosmolar stimulation of mast cells
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- Gulliksson, Magdalena (författare)
- Karolinska Institutet
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- Palmberg, Lena (författare)
- Karolinska Institutet
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- Nilsson, G (författare)
- Karolinska Institutet
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- Ahlstedt, S (författare)
- Karolinska Institutet
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- Kumlin, Maria (författare)
- Sophiahemmet Högskola
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(creator_code:org_t)
- Wiley, 2006
- 2006
- Engelska.
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Ingår i: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 61:12, s. 1473-9
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
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- BACKGROUND: Mannitol-induced bronchoconstriction in subjects with exercise-induced asthma is associated with increased urinary excretion of 9alpha, 11beta-PGF(2), a metabolite of prostaglandin D(2) (PGD(2)) serving as a mast cell marker. It has however been questioned whether or not human mast cells release PGD(2) and leukotriene C(4) (LTC(4)) after osmotic challenge with mannitol in vitro. METHODS: Cord blood-derived human mast cells were stimulated osmotically, immunologically or with a combination of both. Supernatants were analysed for PGD(2), LTC(4) and histamine contents with enzyme immunoassays. RESULTS: Significant release of de novo synthesized eicosanoids, predominantly PGD(2) [12 (8.8, 14) pmol/10(6)cells; median (25th, 75th percentile) but also LTC(4) (0.1 (0.08, 0.15) pmol/10(6) cells] were found in mast cells in vitro in response to 0.7 M mannitol stimulation. A massive release of histamine [70 (5.3)% of total; mean (SEM)] was also found. There were no correlations between the levels of released mediators after mannitol stimulation. In contrast, there was a correlation between release of PGD(2) and LTC(4), following immunological stimulation. CONCLUSION: The findings support that hyperosmolar challenge activates mast cells, but different than antigen stimulation.
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- MEDICIN
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