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In situ mutation de...
In situ mutation detection and visualization of intratumor heterogeneity for cancer research and diagnostics
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Grundberg, Ida (author)
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- Kiflemariam, Sara (author)
- Uppsala universitet,Genomik,Science for Life Laboratory, SciLifeLab
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- Mignardi, Marco (author)
- Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Imgenberg-Kreuz, Juliana (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi
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- Edlund, Karolina (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Botling
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- Micke, Patrick (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Botling
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- Sundström, Magnus (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Botling
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- Sjöblom, Tobias (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Sjöblom
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- Botling, Johan (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Botling
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- Nilsson, Mats (author)
- Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Science for Life Laboratory, SciLifeLab,Molekylära verktyg
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(creator_code:org_t)
- 2013-11-21
- 2013
- English.
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In: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 4:12, s. 2407-2418
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https://doi.org/10.1...
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http://www.oncotarge...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Current assays for somatic mutation analysis are based on extracts from tissue sections that often contain morphologically heterogeneous neoplastic regions with variable contents of genetically normal stromal and inflammatory cells, obscuring the results of the assays. We have developed an RNA-based in situ mutation assay that targets oncogenic mutations in a multiplex fashion that resolves the heterogeneity of the tissue sample. Activating oncogenic mutations are targets for a new generation of cancer drugs. For anti-EGFR therapy prediction, we demonstrate reliable in situ detection of KRAS mutations in codon 12 and 13 in colon and lung cancers in three different types of routinely processed tissue materials. High-throughput screening of KRAS mutation status was successfully performed on a tissue microarray. Moreover, we show how the patterns of expressed mutated and wild-type alleles can be studied in situ in tumors with complex combinations of mutated EGFR, KRAS and TP53. This in situ method holds great promise as a tool to investigate the role of somatic mutations during tumor progression and for prediction of response to targeted therapy.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- Padlock probes
- RCA
- in situ
- KRAS
- cancer diagnostics
- Pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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Oncotarget
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To the university's database
- By the author/editor
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Grundberg, Ida
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Kiflemariam, Sar ...
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Mignardi, Marco
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Imgenberg-Kreuz, ...
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Edlund, Karolina
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Micke, Patrick
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show more...
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Sundström, Magnu ...
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Sjöblom, Tobias
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Botling, Johan
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Nilsson, Mats
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
- Articles in the publication
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Oncotarget
- By the university
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Stockholm University
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Uppsala University