Cellular proton dynamics in Elodea canadensis leaves induced by cadmium

• Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+](cyt) was also investigated as the [Ca2+](cyt) and [pH](cyt) homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+](cyt) and [PH](cyt) followed similar dynamics. Cadmium (0.5 mu M) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol.

Ämnesord

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

Nyckelord

Apoplastic pH

Cadmium

Cytosolic Ca2

Cytosolic pH

Proton dynamics

Protoplast

Vacuolar pH

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