WFRF:(Von Stedingk Henrik)
Search: WFRF:(Von Stedingk Henrik) > LC–MS/MS Screening ...
- 3 of 15
- Previous record
- Next record
- To hitlist
- Carlsson, HenrikStockholms universitet,Institutionen för material- och miljökemi (MMK) (author)
LC–MS/MS Screening Strategy for Unknown Adducts to N-Terminal Valine in Hemoglobin Applied to Smokers and Nonsmokers
- Article/chapterEnglish2014
Publisher, publication year, extent ...
- 2014-11-06
- American Chemical Society (ACS),2014
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:su-112092
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-112092URI
- https://doi.org/10.1021/tx5002749DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Electrophilically reactive compounds have the ability to form adducts with nucleophilic sites in DNA and proteins, constituting a risk for toxic effects. Mass spectrometric detection of adducts to N-terminal valine in hemoglobin (Hb) after detachment by modified Edman degradation procedures is one approach for in vivo monitoring of exposure to electrophilic compounds/metabolites. So far, applications have been limited to one or a few selected reactive species, such as acrylamide and its metabolite glycidamide. This article presents a novel screening strategy for unknown Hb adducts to be used as a basis for an adductomic approach. The method is based on a modified Edman procedure, FIRE, specifically developed for LC-MS/MS analysis of N-terminal valine adducts in Hb detached as fluorescein thiohydantoin (FTH) derivatives. The aim is to detect and identify a priori unknown Hb adducts in human blood samples. Screening of valine adducts was performed by stepwise scanning of precursor ions in small mass increments, monitoring four fragments common for the FTH derivative of valine with different N-substitutions in the multiple-reaction mode, covering a mass range of 135 Da (m/z 503-638). Samples from six smokers and six nonsmokers were analyzed. Control experiments were performed to compare these results with known adducts and to check for artifactual formation of adducts. In all samples of smokers and nonsmokers, seven adducts were identified, of which six have previously been studied. Nineteen unknown adducts were observed, and 14 of those exhibited fragmentation patterns similar to earlier studied FTH derivatives of adducts to valine. Identification of the unknown adducts will be the focus of future work. The presented methodology is a promising screening tool using Hb adducts to indicate exposure to potentially toxic electrophilic compounds and metabolites.
Subject headings and genre
- NATURVETENSKAP Kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences hsv//eng
- Environmental Chemistry
- miljökemi
Added entries (persons, corporate bodies, meetings, titles ...)
- von Stedingk, HansStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)hvons (author)
- Nilsson, UlrikaStockholms universitet,Institutionen för analytisk kemi(Swepub:su)unils (author)
- Törnqvist, MargaretaStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)mt (author)
- Stockholms universitetInstitutionen för material- och miljökemi (MMK) (creator_code:org_t)
Related titles
- In:Chemical Research in Toxicology: American Chemical Society (ACS)27:12, s. 2062-20700893-228X1520-5010
Internet link
Find in a library
- Chemical Research in Toxicology (Search for host publication in LIBRIS)
To the university's database
- 3 of 15
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Carlsson, Henrik
- von Stedingk, Ha ...
- Nilsson, Ulrika
- Törnqvist, Marga ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- Articles in the publication
- Chemical Researc ...
- By the university
- Stockholm University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
