Molecular architecture of the active mitochondrial protein gate

Mitochondria fulfill central functions in cellular energetics, metabolism, and signaling. The outer membrane translocator complex (the TOM complex) imports most mitochondrial proteins, but its architecture is unknown. Using a cross-linking approach, we mapped the active translocator down to single amino acid residues, revealing different transport paths for preproteins through the Tom40 channel. An N-terminal segment of Tom40 passes from the cytosol through the channel to recruit chaperones fromthe intermembrane space that guide the transfer of hydrophobic preproteins. The translocator contains three Tom40 beta-barrel channels sandwiched between a central alpha-helical Tom22 receptor cluster and external regulatory Tom proteins. The preprotein-translocating trimeric complex exchanges with a dimeric isoform to assemble new TOM complexes. Dynamic coupling of alpha-helical receptors, beta-barrel channels, and chaperones generates a versatile machinery that transports about 1000 different proteins.

Imai, Kenichiro (author)
Qiu, Jian (author)
Hewitt, Victoria L. (author)
Tan, Khershing (author)
Shen, Hsin-Hui (author)
Sakiyama, Noriyuki (author)
Fukasawa, Yoshinori (author)
Hayat, SikanderStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab) (author)
Kamiya, Megumi (author)
Elofsson, ArneStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)aelof (author)
Tomii, Kentaro (author)
Horton, Paul (author)
Wiedemann, Nils (author)
Pfanner, Nikolaus (author)
Lithgow, Trevor (author)
Endo, Toshiya (author)
Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)

In:Science: American Association for the Advancement of Science (AAAS)349:6255, s. 1544-15480036-80751095-9203

By the author/editor: Shiota, Takuya; Imai, Kenichiro; Qiu, Jian; Hewitt, Victoria ...; Tan, Khershing; Shen, Hsin-Hui; show more...; Sakiyama, Noriyu ...; Fukasawa, Yoshin ...; Hayat, Sikander; Kamiya, Megumi; Elofsson, Arne; Tomii, Kentaro; Horton, Paul; Wiedemann, Nils; Pfanner, Nikolau ...; Lithgow, Trevor; Endo, Toshiya; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.