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A Flowchart to Anal...
A Flowchart to Analyze Protease Activity in Plant Mitochondria
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- Teixeira, Pedro F. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Branca, Rui M. (author)
- Karolinska Institutet
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- Kmiec, Beata (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Glaser, Elzbieta (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- New York, NY : Springer-Verlag New York, 2015
- 2015
- English.
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In: Plant Mitochondria. - New York, NY : Springer-Verlag New York. - 9781493926398 - 9781493926381 ; 1305, s. 123-30
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- Proteases are one of the most abundant classes of enzymes and are involved in a plethora of biological processes in many cellular compartments, including the mitochondria. To understand the role of proteases is essential to determine their substrate repertoire, preferably in an in vivo setting. In this chapter we describe general guidelines to analyze protease activity using several strategies, from in-gel analysis to mass spectrometry mapping of the cleavage site(s) and fluorogenic probes that can easily be used in vivo. To exemplify this flowchart, we used the recently characterized organellar oligopeptidase of Arabidopsis (Arabidopsis thaliana), an enzyme that takes part in degradation of short peptides within mitochondria and chloroplasts.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Peptide
- Protease
- Mitochondria
- Electrophoresis
- Mass spectrometry
- Fluorogenic probe
Publication and Content Type
- ref (subject category)
- kap (subject category)
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