WFRF:(Branca Rui M. M.)
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- Teixeira, Pedro F.Stockholms universitet,Institutionen för biokemi och biofysik (author)
A Flowchart to Analyze Protease Activity in Plant Mitochondria
- Article/chapterEnglish2015
Publisher, publication year, extent ...
- New York, NY :Springer-Verlag New York,2015
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:su-125889
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-125889URI
- https://doi.org/10.1007/978-1-4939-2639-8_8DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:225910730URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Proteases are one of the most abundant classes of enzymes and are involved in a plethora of biological processes in many cellular compartments, including the mitochondria. To understand the role of proteases is essential to determine their substrate repertoire, preferably in an in vivo setting. In this chapter we describe general guidelines to analyze protease activity using several strategies, from in-gel analysis to mass spectrometry mapping of the cleavage site(s) and fluorogenic probes that can easily be used in vivo. To exemplify this flowchart, we used the recently characterized organellar oligopeptidase of Arabidopsis (Arabidopsis thaliana), an enzyme that takes part in degradation of short peptides within mitochondria and chloroplasts.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
- Peptide
- Protease
- Mitochondria
- Electrophoresis
- Mass spectrometry
- Fluorogenic probe
Added entries (persons, corporate bodies, meetings, titles ...)
- Branca, Rui M.Karolinska Institutet (author)
- Kmiec, BeataStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)bkmie (author)
- Glaser, ElzbietaStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)glase (author)
- Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)
Related titles
- In:Plant MitochondriaNew York, NY : Springer-Verlag New York1305, s. 123-3097814939263989781493926381
- In:Methods in molecular biology (Clifton, N.J.)New York, NY : Springer-Verlag New York1305, s. 123-301940-6029
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