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Distribution of loc...
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Ruokonen, Suvi-Katriina
(author)
Distribution of local anesthetics between aqueous and liposome phases
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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Elsevier BV,2017
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-140220
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-140220URI
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https://doi.org/10.1016/j.chroma.2016.12.005DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Liposomes were used as biomimetic models in capillary electrokinetic chromatography (EKC) for the determination of distribution constants (K-D) of certain local anesthetics and a commonly used preservative. Synthetic liposomes comprised phosphatidylcholine and phosphatidylglycerol phospholipids with and without cholesterol. In addition, ghost liposomes made from red blood cell (RBC) lipid extracts were used as pseudostationary phase to acquire information on how the liposome composition affects the interactions between anesthetics and liposomes. These results were compared with theoretical distribution coefficients at pH 7.4. In addition to 25 degrees C, the distribution constants were determined at 37 and 42 degrees C to simulate physiological conditions. Moreover, the usability of five electroosmotic flow markers in liposome (LEKC) and micellar EKC (MEKC) was studied. LEKC was proven to be a convenient and fast technique for obtaining data about the distribution constants of local anesthetics between liposome and aqueous phase. RBC liposomes can be utilized for more representative model of cellular membranes, and the results indicate that the distribution constants of the anesthetics are greatly dependent on the used liposome composition and the amount of cholesterol, while the effect of temperature on the distribution constants is less significant.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Dusa, Filip
(author)
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Rantamaki, Antti H.
(author)
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Robciuc, Alexandra
(author)
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Holma, Paula
(author)
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Holopainen, Juha M.
(author)
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Abdel-Rehim, MohamedStockholms universitet,Institutionen för miljövetenskap och analytisk kemi(Swepub:su)rabde
(author)
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Wiedmer, Susanne K.
(author)
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Stockholms universitetInstitutionen för miljövetenskap och analytisk kemi
(creator_code:org_t)
Related titles
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In:Journal of Chromatography A: Elsevier BV1479, s. 194-2030021-96731873-3778
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