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Macrophage activati...
Macrophage activation status determines the internalization of mesoporous silica particles of different sizes : Exploring the role of different pattern recognition receptors
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- Gallud, Audrey (author)
- Karolinska Institutet
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Bondarenko, Olesja (author)
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- Feliu, Neus (author)
- Karolinska Institutet
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- Kupferschmidt, Natalia (author)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
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Atluri, Rambabu (author)
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Garcia-Bennett, Alfonso (author)
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- Fadeel, Bengt (author)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 121, s. 28-40
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- Mesoporous silica-based particles are promising candidates for biomedical applications. Here, we address the importance of macrophage activation status for internalization of AMS6 (approx. 200 nm in diameter) versus AMS8 (approx. 2 mu m) mesoporous silica particles and the role of different phagocytosis receptors for particle uptake. To this end, FITC-conjugated silica particles were used. AMS8 were found to be non-cytotoxic both for M-CSF-stimulated (anti-inflammatory) and GM-CSF-stimulated (pro-inflammatory) macrophages, whereas AMS6 exhibited cytotoxicity towards M-CSF-stimulated, but not GMCSF-stimulated macrophages; this toxicity was, however, mitigated in the presence of serum. AMS8 triggered the secretion of pro-inflammatory cytokines in M-CSF-activated cells. Class A scavenger receptor (SR-A) expression was noted in both M-CSF and GM-CSF-stimulated macrophages, although the expression was higher in the former case, and gene silencing of SR-A resulted in a decreased uptake of AMS6 in the absence of serum. GM-CSF-stimulated macrophages expressed higher levels of the mannose receptor CD206 compared to M-CSF-stimulated cells, and uptake of AMS6, but not AMS8, was reduced following the downregulation of CD206 in GM-CSF-stimulated cells; particle uptake was also suppressed by mannan, a competitive ligand. These studies demonstrate that macrophage activation status is an important determinant of particle uptake and provide evidence for a role of different macrophage receptors for cell uptake of silica particles.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering (hsv//eng)
Keyword
- Silica particles
- Human macrophages
- Scavenger receptors
- mannose receptors
Publication and Content Type
- ref (subject category)
- art (subject category)
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