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- Tzafestas, Kyriakos (author)
Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2016-12-06
- Wiley,2017
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:su-142613
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-142613URI
- https://doi.org/10.1111/nph.14326DOI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4, 6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax), and five-fold lower Michaelis Constant (Km) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites.
Subject headings and genre
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- 2
- 4
- 6-trinitrotoluene
- Arabidopsis
- conjugation
- detoxification
- Drosophila melanogaster
- environmental pollutant
- glutathione transferase
- phytoremediation
Added entries (persons, corporate bodies, meetings, titles ...)
- Razalan, Maria M. (author)
- Gyulev, Ivan (author)
- Mazari, Aslam M. A.Stockholms universitet,Institutionen för neurokemi (author)
- Mannervik, BengtStockholms universitet,Institutionen för neurokemi(Swepub:su)bmann (author)
- Rylott, Elizabeth L. (author)
- Bruce, Neil C. (author)
- Stockholms universitetInstitutionen för neurokemi (creator_code:org_t)
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- In:New Phytologist: Wiley214:1, s. 294-3030028-646X1469-8137
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