Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation

Olsen, Jessica M.Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut (author)

Brown adipose tissue is the primary site for thermogenesis and can consume, in addition to free fatty acids, a very high amount of glucose from the blood, which can both acutely and chronically affect glucose homeostasis. Here, we show that mechanistic target of rapamycin (mTOR) complex 2 has a novel role in β3-adrenoceptor-stimulated glucose uptake in brown adipose tissue. We show that β3-adrenoceptors stimulate glucose uptake in brown adipose tissue via a signaling pathway that is comprised of two different parts: one part dependent on cAMP-mediated increases in GLUT1 transcription and de novo synthesis of GLUT1 and another part dependent on mTOR complex 2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane, leading to increased glucose uptake. Both parts are essential for β3-adrenoceptor-stimulated glucose uptake. Importantly, the effect of β3-adrenoceptor on mTOR complex 2 is independent of the classical insulin-phosphoinositide 3-kinase-Akt pathway, highlighting a novel mechanism of mTOR complex 2 activation.

Sato, MasaakiStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Monash Institute of Pharmaceutical Sciences, Australia (author)
Dallner, Olof S.Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,The Rockefeller University, USA (author)
Sandström, Anna L.Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)ansa2665 (author)
Pisani, Didier F. (author)
Jean-Claude, Chambard (author)
Amri, Ez-Zoubir (author)
Hutchinson, Dana S. (author)
Bengtsson, ToreStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)tbeng (author)
Stockholms universitetInstitutionen för molekylär biovetenskap, Wenner-Grens institut (creator_code:org_t)

By the author/editor: Olsen, Jessica M ...; Sato, Masaaki; Dallner, Olof S.; Sandström, Anna ...; Pisani, Didier F ...; Jean-Claude, Cha ...; show more...; Amri, Ez-Zoubir; Hutchinson, Dana ...; Bengtsson, Tore; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

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