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Conjugative type IV...
Conjugative type IV secretion in Gram-positive pathogens : TraG, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein TraM
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- Kohler, Verena, 1992- (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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- Probst, Ines (författare)
- Division of Infectious Diseases, Department of Internal Medicine, University Medical Center Freiburg, Freiburg, Germany; Faculty of Biology, Microbiology, Albert-Ludwigs-University Freiburg, Freiburg, Germany
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Aufschnaiter, Andreas (författare)
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- Büttner, Sabrina (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Institute of Molecular Biosciences, University of Graz, Graz, Austria; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
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- Schaden, Lisa (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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- Rechberger, Gerald N. (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria; Omics Center Graz, BioTechMed-Graz, Graz, Austria
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- Koraimann, Günther (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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- Grohmann, Elisabeth (författare)
- Division of Infectious Diseases, Department of Internal Medicine, University Medical Center Freiburg, Freiburg, Germany; Faculty of Life Sciences and Technology, Beuth University of Applied Sciences, Berlin, Germany
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- Keller, Walter (författare)
- Division of Infectious Diseases, Department of Internal Medicine, University Medical Center Freiburg, Freiburg, Germany; Faculty of Life Sciences and Technology, Beuth University of Applied Sciences, Berlin, Germany
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- Kohler, Andreas, Dr. rer. nat. 1988- (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- Engelska.
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Ingår i: Plasmid. - : Elsevier BV. - 0147-619X .- 1095-9890. ; 91, s. 9-18
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Grampositive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 transfer genes. In this work, we focus on the VirB1-like protein TraG, a modular peptidoglycan metabolizing enzyme, and the VirB8-homolog TraM, a potential member of the translocation channel. By providing full-length traG in trans, but not with a truncated variant, we achieved full recovery of wild type transfer efficiency in the traG-knockout mutant E. faecalis pIP501AtraG. With peptidoglycan digestion experiments and tandem mass spectrometry we could assign lytic transglycosylase and endopeptidase activity to TraG, with the CHAP domain alone displaying endopeptidase activity. We identified a novel interaction between TraG and TraM in a bacterial 2-hybrid assay. In addition we found that both proteins localize in focal spots at the E. faecalis cell membrane using immunostaining and fluorescence microscopy. Extracellular protease digestion to evaluate protein cell surface exposure revealed that correct membrane localization of TraM requires the transmembrane helix of TraG. Thus, we suggest an essential role for TraG in the assembly of the pIP501 type IV secretion system.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Type IV secretion system
- Enterococcus faecalis
- Lytic transglycosylase
- Endopeptidase
- VirB1
- VirB8
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Kohler, Verena, ...
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Probst, Ines
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Aufschnaiter, An ...
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Büttner, Sabrina
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Schaden, Lisa
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Rechberger, Gera ...
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Koraimann, Günth ...
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Grohmann, Elisab ...
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Keller, Walter
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