Disease detection : Volatile biomarkers in acute inflammation

• Through history, infectious bacteria and viruses have posed a threat to humanity. Being able to detect and avoid pathogens is, therefore, of crucial importance. It has been shown that body odor samples, such as urine, from immune-activated animals contain sickness cues and detection of which, results in avoidance behavior in conspecifics. Perceivable changes in body odor samples have also, recently, been shown in immune-activated human participants. The main aim of this study was to identify potential volatile biomarkers of the acute inflammatory response. Healthy volunteers were injected twice in a crossover design, once with the bacterial endotoxin lipopolysaccharide (LPS, 2ng/kg bw) and once with placebo (saline). LPS caused a transient systemic inflammatory response as shown by pro-inflammatory cytocines, tympanic temperature and subjective sickness ratings (significant interactions between condition and time with all ps<.001, and all ηρ2>.663). Axillary sweat and urine were collected both before and 2–4 hours after injection. Headspace from these samples were analyzed using gas chromatography-mass spectrometry (GC-MS). GC-MS data analyses assessed the differences in the profile of volatile compounds of urine and sweat from LPS and placebo donors. Results regarding possible differences between volatile biomarkers in LPS and placebo condition will be presented and discussed.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Neurologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Neurology (hsv//eng)

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