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Targeted lipidomics...
Targeted lipidomics analysis identified altered serum lipid profiles in patients with polymyositis and dermatomyositis
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Raouf, Joan (författare)
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- Idborg, Helena (författare)
- Karolinska Institutet
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- Englund, Petter (författare)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
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- Alexanderson, Helene (författare)
- Karolinska Institutet
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Dastmalchi, Maryam (författare)
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- Jakobsson, Per-Johan (författare)
- Karolinska Institutet
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- Lundberg, Ingrid E. (författare)
- Karolinska Institutet
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- Korotkova, Marina (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2018-05-02
- 2018
- Engelska.
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Ingår i: Arthritis Research & Therapy. - : Springer Science and Business Media LLC. - 1478-6362. ; 20
- Relaterad länk:
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https://doi.org/10.1...
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https://arthritis-re...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Polymyositis (PM) and dermatomyositis (DM) are severe chronic autoimmune diseases, characterized by muscle fatigue and low muscle endurance. Conventional treatment includes high doses of glucocorticoids and immunosuppressive drugs; however, few patients recover full muscle function. One explanation of the persistent muscle weakness could be altered lipid metabolism in PM/DM muscle tissue as we previously reported. Using a targeted lipidomic approach we aimed to characterize serum lipid profiles in patients with PM/DM compared to healthy individuals (HI) in a cross-sectional study. Also, in the longitudinal study we compared serum lipid profiles in patients newly diagnosed with PM/DM before and after immunosuppressive treatment. Methods: Lipidomic profiles were analyzed in serum samples from 13 patients with PM/DM, 12 HI and 8 patients newly diagnosed with PM/DM before and after conventional immunosuppressive treatment using liquid chromatography tandem mass spectrometry (LC-MS/MS) and a gas-chromatography flame ionization detector (GC-FID). Functional Index (FI), as a test of muscle performance and serum levels of creatine kinase (s-CK) as a proxy for disease activity were analyzed. Results: The fatty acid (FA) composition of total serum lipids was altered in patients with PM/DM compared to HI; the levels of palmitic (16: 0) acid were significantly higher while the levels of arachidonic (20: 4, n-6) acid were significantly lower in patients with PM/DM. The profiles of serum phosphatidylcholine and triacylglycerol species were changed in patients with PM/DM compared to HI, suggesting disproportionate levels of saturated and polyunsaturated FAs that might have negative effects on muscle performance. After immunosuppressive treatment the total serum lipid levels of eicosadienoic (20: 2, n-6) and eicosapentaenoic (20: 5, n-3) acids were increased and serum phospholipid profiles were altered in patients with PM/DM. The correlation between FI or s-CK and levels of several lipid species indicate the important role of lipid changes in muscle performance and inflammation. Conclusions: Serum lipids profiles are significantly altered in patients with PM/DM compared to HI. Moreover, immunosuppressive treatment in patients newly diagnosed with PM/DM significantly affected serum lipid profiles. These findings provide new evidence of the dysregulated lipid metabolism in patients with PM/DM that could possibly contribute to low muscle performance.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Lipidomics
- Fatty acids
- Phospholipids
- Polymyositis
- Dermatomyositis
- Immunosuppressive treatment
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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