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Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
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- Nordin, Joel Z. (author)
- Karolinska Institutet
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- Lee, Yi (author)
- Karolinska Institutet
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Vader, Pieter (author)
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Mäger, Imre (author)
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- Johansson, Henrik J. (author)
- Karolinska Institutet
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Heusermann, Wolf (author)
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- Wiklander, Oscar P. B. (author)
- Karolinska Institutet
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- Hällbrink, Mattias (author)
- Stockholms universitet,Institutionen för neurokemi
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Seow, Yiqi (author)
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Bultema, Jarred J. (author)
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- Gilthorpe, Jonathan (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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Davies, Tim (author)
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Fairchild, Paul J. (author)
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- Gabrielsson, Susanne (author)
- Karolinska Institutet
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Meisner-Kober, Nicole C. (author)
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- Lehtio, Janne (author)
- Karolinska Institutet
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- Smith, C. I. Edvard (author)
- Karolinska Institutet
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Wood, Matthew J. A. (author)
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- Andaloussi, Samir E. L. (author)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Nanomedicine. - : Elsevier BV. - 1549-9634 .- 1549-9642. ; 11:4, s. 879-883
- Related links:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading toadifferent in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Biophysical properties
- extracellular vesicles
- size-exclusion liquid chromatography
- ultracentrifugation
- ultrafiltration
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Nordin, Joel Z.
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Lee, Yi
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Vader, Pieter
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Mäger, Imre
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Johansson, Henri ...
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Heusermann, Wolf
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show more...
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Wiklander, Oscar ...
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Hällbrink, Matti ...
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Seow, Yiqi
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Bultema, Jarred ...
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Gilthorpe, Jonat ...
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Davies, Tim
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Fairchild, Paul ...
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Gabrielsson, Sus ...
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Meisner-Kober, N ...
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Lehtio, Janne
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Smith, C. I. Edv ...
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Wood, Matthew J. ...
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Andaloussi, Sami ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Nanomedicine
- By the university
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Stockholm University
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Umeå University
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Karolinska Institutet