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New tools for autom...
New tools for automated high-resolution cryo-EM structure determination in RELION-3
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- Zivanov, Jasenko (författare)
- MRC, Lab Mol Biol, Cambridge, England.
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- Nakane, Takanori (författare)
- MRC, Lab Mol Biol, Cambridge, England.
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- Forsberg, Björn O. (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden.
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- Kimanius, Dari (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden.
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- Hagen, Wim J. H. (författare)
- European Mol Biol Lab, Struct & Computat Biol Unit, Heidelberg, Germany.;European Mol Biol Lab, Cryoelectron Microscopy Serv Platform, Heidelberg, Germany.
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- Lindahl, Erik (författare)
- KTH,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),KTH Royal Institute of Technology, Sweden,SeRC - Swedish e-Science Research Centre,Science for Life Laboratory, SciLifeLab,Tillämpad fysik,Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden.
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- Scheres, Sjors H. W. (författare)
- MRC, Lab Mol Biol, Cambridge, England.
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MRC, Lab Mol Biol, Cambridge, England Institutionen för biokemi och biofysik (creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2018
- 2018
- Engelska.
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Ingår i: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 7
- Relaterad länk:
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https://doi.org/10.7...
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https://urn.kb.se/re...
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https://doi.org/10.7...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 angstrom compared to previous RELION versions.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Biochemistry towards Bioinformatics
- biokemi med inriktning mot bioinformatik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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