Search: id:"swepub:oai:DiVA.org:su-166028" >
Nutritional Control...
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Leslie, David J.Philipps University Marburg, Germany; Max Planck Institute for Terrestrial Microbiology, Germany
(author)
Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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2015-07-02
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Public Library of Science (PLoS),2015
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-166028
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-166028URI
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https://doi.org/10.1371/journal.pgen.1005342DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Bacteria can arrest their own growth and proliferation upon nutrient depletion and under various stressful conditions to ensure their survival. However, the molecular mechanisms responsible for suppressing growth and arresting the cell cycle under such conditions remain incompletely understood. Here, we identify post-transcriptional mechanisms that help enforce a cell-cycle arrest in Caulobacter crescentus following nutrient limitation and during entry into stationary phase by limiting the accumulation of DnaA, the conserved replication initiator protein. DnaA is rapidly degraded by the Lon protease following nutrient limitation. However, the rate of DnaA degradation is not significantly altered by changes in nutrient availability. Instead, we demonstrate that decreased nutrient availability downregulates dnaA translation by a mechanism involving the 5' untranslated leader region of the dnaA transcript; Lon-dependent proteolysis of DnaA then outpaces synthesis, leading to the elimination of DnaA and the arrest of DNA replication. Our results demonstrate how regulated translation and constitutive degradation provide cells a means of precisely and rapidly modulating the concentration of key regulatory proteins in response to environmental inputs.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Heinen, ChristianPhilipps University Marburg, Germany, Max Planck Institute for Terrestrial Microbiology, Germany
(author)
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Schramm, Frederic D.Philipps University Marburg, Germany(Swepub:su)fresc
(author)
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Thüring, MariettaPhilipps University Marburg, Germany
(author)
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Aakre, Christopher D.Massachusetts Institute of Technology, United States of America
(author)
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Murray, Sean M.Philipps University Marburg, Germany; Max Planck Institute for Terrestrial Microbiology, Germany
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Laub, Michael T.Massachusetts Institute of Technology, United States of America
(author)
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Jonas, KristinaPhilipps University Marburg, Germany(Swepub:su)krijo
(author)
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Philipps University Marburg, Germany; Max Planck Institute for Terrestrial Microbiology, GermanyPhilipps University Marburg, Germany, Max Planck Institute for Terrestrial Microbiology, Germany
(creator_code:org_t)
Related titles
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In:PLOS Genetics: Public Library of Science (PLoS)11:71553-73901553-7404
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