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YtrA(Sa), a GntR-Fa...
YtrA(Sa), a GntR-Family Transcription Factor, Represses Two Genetic Loci Encoding Membrane Proteins in Sulfolobus acidocaldarius
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- Lemmens, Liesbeth (författare)
- Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Brussels, Belgium
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- Tilleman, Laurentijn (författare)
- Univ Ghent, Fac Pharmaceut Sci, Lab Pharmaceut Biotechnol, Ghent, Belgium
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- De Koning, Ezra (författare)
- Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Brussels, Belgium
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- Valegård, Karin (författare)
- Uppsala universitet,Molekylär biofysik
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- Lindås, Ann-Christin (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden
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- Van Nieuwerburgh, Filip (författare)
- Univ Ghent, Fac Pharmaceut Sci, Lab Pharmaceut Biotechnol, Ghent, Belgium
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- Maes, Dominique (författare)
- Vrije Univ Brussel, Dept Bioengn Sci, Struct Biol Brussels, Brussels, Belgium
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- Peeters, Eveline (författare)
- Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Brussels, Belgium
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(creator_code:org_t)
- 2019-09-10
- 2019
- Engelska.
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Ingår i: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 10
- Relaterad länk:
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Abstract
Ämnesord
Stäng
- In bacteria, the GntR family is a widespread family of transcription factors responsible for the regulation of a myriad of biological processes. In contrast, despite their occurrence in archaea only a little information is available on the function of GntR-like transcription factors in this domain of life. The thermoacidophilic crenarchaeon Sulfolobus acidocaldarius harbors a GntR-like regulator belonging to the YtrA subfamily, encoded as the first gene in an operon with a second gene encoding a putative membrane protein. Here, we present a detailed characterization of this regulator, named YtrA(Sa), with a focus on regulon determination and mechanistic analysis with regards to DNA binding. Genome-wide chromatin immunoprecipitation and transcriptome experiments, the latter employing a ytrA(Sa) overexpression strain, demonstrate that the regulator acts as a repressor on a very restricted regulon, consisting of only two targets including the operon encoding its own gene and a distinct genetic locus encoding another putative membrane protein. For both targets, a conserved 14-bp semi-palindromic binding motif was delineated that covers the transcriptional start site and that is surrounded by additional half-site motifs. The crystallographic structure of YtrA(Sa) was determined, revealing a compact dimeric structure in which the DNA-binding motifs are oriented ideally to enable a specific high-affinity interaction with the core binding motif. This study provides new insights into the functioning of a YtrA-like regulator in the archaeal domain of life.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- archaea
- Sulfolobus
- transcription regulation
- GntR
- YtrA
- membrane protein
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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