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A Mass-Spectrometry...
A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins
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Bolla, Jani Reddy (author)
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Corey, Robin A. (author)
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- Sahin, Cagla (author)
- Karolinska Institutet
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Gault, Joseph (author)
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Hummer, Alissa (author)
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Hopper, Jonathan T. S. (author)
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Lane, David P. (author)
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- Drew, David (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Allison, Timothy M. (author)
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Stansfeld, Phillip J. (author)
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Robinson, Carol (author)
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- Landreh, Michael (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2020-01-29
- 2020
- English.
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In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 59:9, s. 3523-3528
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Abstract
Subject headings
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- Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically bound lipids, and non-specific, annular interactions is a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid-binding properties, we explore the ability of detergents to compete with lipids bound in different environments. We show that lipids in annular positions on the presenilin homologue protease are subject to constant exchange with detergent. By contrast, detergent-resistant lipids bound at the dimer interface in the leucine transporter show decreased k(off) rates in molecular dynamics simulations. Turning to the lipid flippase MurJ, we find that addition of the natural substrate lipid-II results in the formation of a 1:1 protein-lipid complex, where the lipid cannot be displaced by detergent from the highly protected active site. In summary, we distinguish annular from non-annular lipids based on their exchange rates in solution.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- lipid binding
- membrane protein structure
- molecular dynamics
- native mass spectrometry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bolla, Jani Redd ...
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Corey, Robin A.
-
Sahin, Cagla
-
Gault, Joseph
-
Hummer, Alissa
-
Hopper, Jonathan ...
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show more...
-
Lane, David P.
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Drew, David
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Allison, Timothy ...
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Stansfeld, Phill ...
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Robinson, Carol
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Landreh, Michael
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Angewandte Chemi ...
- By the university
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Stockholm University
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Karolinska Institutet