Search: id:"swepub:oai:DiVA.org:su-181762" >
Conformational dyna...
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Mader, Sophie L.
(author)
Conformational dynamics modulate the catalytic activity of the molecular chaperone Hsp90
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-03-16
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Springer Science and Business Media LLC,2020
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-181762
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-181762URI
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https://doi.org/10.1038/s41467-020-15050-0DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The heat shock protein 90 (Hsp90) is a molecular chaperone that employs the free energy of ATP hydrolysis to control the folding and activation of several client proteins in the eukaryotic cell. To elucidate how the local ATPase reaction in the active site couples to the global conformational dynamics of Hsp90, we integrate here large-scale molecular simulations with biophysical experiments. We show that the conformational switching of conserved ion pairs between the N-terminal domain, harbouring the active site, and the middle domain strongly modulates the catalytic barrier of the ATP-hydrolysis reaction by electrostatic forces. Our combined findings provide a mechanistic model for the coupling between catalysis and protein dynamics in Hsp90, and show how long-range coupling effects can modulate enzymatic activity.
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Lopez, Abraham
(author)
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Lawatscheck, Jannis
(author)
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Luo, Qi
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Rutz, Daniel A.
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Gamiz-Hernandez, Ana P.Stockholms universitet,Institutionen för biokemi och biofysik,Technical University of Munich, Germany(Swepub:su)anga3525
(author)
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Sattler, Michael
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Buchner, Johannes
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Kaila, Ville R. I.Stockholms universitet,Institutionen för biokemi och biofysik,Technical University of Munich, Germany(Swepub:su)vika1812
(author)
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Stockholms universitetInstitutionen för biokemi och biofysik
(creator_code:org_t)
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In:Nature Communications: Springer Science and Business Media LLC11:12041-1723
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