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Predicting sample s...
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Keighley, XéniaUniversity of Groningen,University of Copenhagen
(författare)
Predicting sample success for large-scale ancient DNA studies on marine mammals
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
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2021-02-26
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Wiley,2021
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-189940
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-189940URI
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https://doi.org/10.1111/1755-0998.13331DOI
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https://lup.lub.lu.se/record/0d60dbaa-b650-46df-80e0-892c0b50020eURI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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In recent years, non-human ancient DNA studies have begun to focus on larger sample sizes and whole genomes, offering the potential to reveal exciting and hitherto unknown answers to ongoing biological and archaeological questions. However, one major limitation to the feasibility of such studies is the substantial financial and time investments still required during sample screening, due to uncertainty regarding successful sample selection. This study investigates the effect of a wide range of sample properties including latitude, sample age, skeletal element, collagen preservation, and context on endogenous content and DNA damage profiles for 317 ancient and historic pinnipedsamples collected from across the North Atlantic. Using generalised linear and mixed-effectmodels, we found that a range of factors affected DNA preservation within each of the species under consideration. The most important findings were that endogenous content varied significantly according to context, the type of skeletal element, the collagen content and collection year. There also appears to be an effect of the sample’s geographic origin, with samples from the Arcticgenerally showing higher endogenous content and lower damage rates. Both latitude and sample age were found to have significant relationships with damage levels, but only for walrus samples. Sex, ontogenetic age and extraction material preparation were not found to have any significantrelationship with DNA preservation. Overall, the skeletal element and sample context were found to be the most influential factors and should therefore be considered when selecting samples for large-scale ancient genome studies.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bro-Jørgensen, Maiken HemmeStockholm University,Stockholms universitet,Arkeologiska forskningslaboratoriet,University of Copenhagen, Denmark(Swepub:su)mbro
(författare)
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Ahlgren, HansStockholm University,Stockholms universitet,Arkeologiska forskningslaboratoriet(Swepub:su)haah7650
(författare)
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Szpak, PaulTrent University
(författare)
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Ciucani, Marta MariaUniversity of Copenhagen
(författare)
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Sánchez Barreiro, FátimaUniversity of Copenhagen
(författare)
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Howse, LesleyUniversity of Toronto
(författare)
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Gotfredsen, Anne BirgitteUniversity of Copenhagen
(författare)
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Glykou, AikateriniStockholm University,Stockholms universitet,Arkeologiska forskningslaboratoriet(Swepub:su)aglyk
(författare)
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Jordan, PeterLund University,Lunds universitet,Arkeologi,Institutionen för arkeologi och antikens historia,Institutioner,Humanistiska och teologiska fakulteterna,Archaeology,Department of Archaeology and Ancient History,Departments,Joint Faculties of Humanities and Theology(Swepub:lu)pe2732jo
(författare)
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Lidén, KerstinStockholm University,Stockholms universitet,Arkeologiska forskningslaboratoriet(Swepub:su)kliden
(författare)
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Olsen, Morten TangeUniversity of Copenhagen
(författare)
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University of GroningenUniversity of Copenhagen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Molecular Ecology Resources: Wiley21:4, s. 1149-11661755-098X1755-0998
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Keighley, Xénia
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Bro-Jørgensen, M ...
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Ahlgren, Hans
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Szpak, Paul
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Ciucani, Marta M ...
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Sánchez Barreiro ...
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visa fler...
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Howse, Lesley
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Gotfredsen, Anne ...
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Glykou, Aikateri ...
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Jordan, Peter
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Lidén, Kerstin
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Olsen, Morten Ta ...
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visa färre...
- Om ämnet
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- HUMANIORA
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HUMANIORA
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och Historia och ark ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Genetik
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Molecular Ecolog ...
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Stockholms universitet
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Lunds universitet