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Inter-laboratory comparison of gene expression biodosimetry for protracted radiation exposures as part of the RENEB and EURADOS WG10 2019 exercise

Abend, M. (författare)
University of Ulm
Amundson, S. A. (författare)
Columbia University,University of Ulm
Badie, C. (författare)
Public Health England
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Brzoska, K. (författare)
Institute of Nuclear Chemistry and Technology
Hargitai, R. (författare)
József Fodor National Center for Public Health
Kriehuber, R. (författare)
Jülich Research Centre
Schüle, S. (författare)
Columbia University
Kis, E. (författare)
National Public Health and Medical Officer Service, Hungary
Ghandhi, S. A. (författare)
Lumniczky, K. (författare)
National Public Health and Medical Officer Service, Hungary
Morton, S. R. (författare)
O'Brien, G. (författare)
Public Health England
Oskamp, D. (författare)
Jülich Research Centre
Ostheim, P. (författare)
University of Ulm
Siebenwirth, C. (författare)
University of Ulm
Shuryak, I. (författare)
Columbia University
Szatmári, T. (författare)
National Public Health and Medical Officer Service, Hungary
Unverricht-Yeboah, M. (författare)
Jülich Research Centre
Ainsbury, E. (författare)
Public Health England
Bassinet, C. (författare)
Institute For Radiological Protection And Nuclear Safety (irsn)
Kulka, U. (författare)
Federal Office for Radiation Protection
Oestreicher, U. (författare)
Federal Office for Radiation Protection
Ristic, Y. (författare)
Institute For Radiological Protection And Nuclear Safety (irsn)
Trompier, F. (författare)
Institute For Radiological Protection And Nuclear Safety (irsn)
Wójcik, Andrzej (författare)
Stockholm University,Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Waldner, L. (författare)
Lund University,Lunds universitet,Medicinsk strålningsfysik, Malmö,Forskargrupper vid Lunds universitet,Medical Radiation Physics, Malmö,Lund University Research Groups
Port, M. (författare)
University of Ulm
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 (creator_code:org_t)
2021-05-07
2021
Engelska.
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Large-scale radiation emergency scenarios involving protracted low dose rate radiation exposure (e.g. a hidden radioactive source in a train) necessitate the development of high throughput methods for providing rapid individual dose estimates. During the RENEB (Running the European Network of Biodosimetry) 2019 exercise, four EDTA-blood samples were exposed to an Iridium-192 source (1.36 TBq, Tech-Ops 880 Sentinal) at varying distances and geometries. This resulted in protracted doses ranging between 0.2 and 2.4 Gy using dose rates of 1.5-40 mGy/min and exposure times of 1 or 2.5 h. Blood samples were exposed in thermo bottles that maintained temperatures between 39 and 27.7 degrees C. After exposure, EDTA-blood samples were transferred into PAXGene tubes to preserve RNA. RNA was isolated in one laboratory and aliquots of four blinded RNA were sent to another five teams for dose estimation based on gene expression changes. Using an X-ray machine, samples for two calibration curves (first: constant dose rate of 8.3 mGy/min and 0.5-8 h varying exposure times; second: varying dose rates of 0.5-8.3 mGy/min and 4 h exposure time) were generated for distribution. Assays were run in each laboratory according to locally established protocols using either a microarray platform (one team) or quantitative real-time PCR (qRT-PCR, five teams). The qRT-PCR measurements were highly reproducible with coefficient of variation below 15% in >= 75% of measurements resulting in reported dose estimates ranging between 0 and 0.5 Gy in all samples and in all laboratories. Up to twofold reductions in RNA copy numbers per degree Celsius relative to 37 degrees C were observed. However, when irradiating independent samples equivalent to the blinded samples but increasing the combined exposure and incubation time to 4 h at 37 degrees C, expected gene expression changes corresponding to the absorbed doses were observed. Clearly, time and an optimal temperature of 37 degrees C must be allowed for the biological response to manifest as gene expression changes prior to running the gene expression assay. In conclusion, dose reconstructions based on gene expression measurements are highly reproducible across different techniques, protocols and laboratories. Even a radiation dose of 0.25 Gy protracted over 4 h (1 mGy/min) can be identified. These results demonstrate the importance of the incubation conditions and time span between radiation exposure and measurements of gene expression changes when using this method in a field exercise or real emergency situation.

Ämnesord

NATURVETENSKAP  -- Annan naturvetenskap (hsv//swe)
NATURAL SCIENCES  -- Other Natural Sciences (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)

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