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Chromosome Level As...
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Celorio-Mancera, Maria de la PazStockholms universitet,Zoologiska institutionen
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Chromosome Level Assembly of the Comma Butterfly (Polygonia c-album)
- Artikel/kapitelEngelska2021
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2021-03-22
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Oxford University Press (OUP),2021
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LIBRIS-ID:oai:DiVA.org:su-196360
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-196360URI
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https://doi.org/10.1093/gbe/evab054DOI
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Språk:engelska
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Sammanfattning på:engelska
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The comma butterfly (Polygonia c-album, Nymphalidae, Lepidoptera) is a model insect species, most notably in the study of phenotypic plasticity and plant-insect coevolutionary interactions. In order to facilitate the integration of genomic tools with a diverse body of ecological and evolutionary research, we assembled the genome of a Swedish comma using 10X sequencing, scaffolding with matepair data, genome polishing, and assignment to linkage groups using a high-density linkage map. The resulting genome is 373 Mb in size, with a scaffold N50 of 11.7 Mb and contig N50 of 11,2Mb. The genome contained 90.1% of single-copy Lepidopteran orthologs in a BUSCO analysis of 5,286 genes. A total of 21,004 gene-models were annotated on the genome using RNA-Seq data from larval and adult tissue in combination with proteins from the Arthropoda database, resulting in a high-quality annotation for which functional annotations were generated. We further documented the quality of the chromosomal assembly via synteny assessment with Melitaea cinxia. The resulting annotated, chromosome-level genome will provide an important resource for investigating coevolutionary dynamics and comparative analyses in Lepidoptera.
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Rastas, Pasi
(författare)
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Steward, Rachel A.Stockholms universitet,Zoologiska institutionen(Swepub:su)rast4916
(författare)
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Nylin, SörenStockholms universitet,Zoologiska institutionen(Swepub:su)snylin
(författare)
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Wheat, Christopher W.Stockholms universitet,Zoologiska institutionen(Swepub:su)cwhea
(författare)
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Stockholms universitetZoologiska institutionen
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Ingår i:Genome Biology and Evolution: Oxford University Press (OUP)13:51759-6653
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