Sökning: onr:"swepub:oai:DiVA.org:su-200948" >
Action spectroscopy...
-
Coughlan, Neville J. A.
(författare)
Action spectroscopy of the isolated red Kaede fluorescent protein chromophore
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
-
AIP Publishing,2021
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:su-200948
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-200948URI
-
https://doi.org/10.1063/5.0063258DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Incorporation of fluorescent proteins into biochemical systems has revolutionized the field of bioimaging. In a bottom-up approach, understanding the photophysics of fluorescent proteins requires detailed investigations of the light-absorbing chromophore, which can be achieved by studying the chromophore in isolation. This paper reports a photodissociation action spectroscopy study on the deprotonated anion of the red Kaede fluorescent protein chromophore, demonstrating that at least three isomers–assigned to deprotomers–are generated in the gas phase. Deprotomer-selected action spectra are recorded over the S1 ← S0 band using an instrument with differential mobility spectrometry coupled with photodissociation spectroscopy. The spectrum for the principal phenoxide deprotomer spans the 480–660 nm range with a maximum response at ≈610 nm. The imidazolate deprotomer has a blue-shifted action spectrum with a maximum response at ≈545 nm. The action spectra are consistent with excited state coupled-cluster calculations of excitation wavelengths for the deprotomers. A third gas-phase species with a distinct action spectrum is tentatively assigned to an imidazole tautomer of the principal phenoxide deprotomer. This study highlights the need for isomer-selective methods when studying the photophysics of biochromophores possessing several deprotonation sites.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Stockett, Mark H.Stockholms universitet,Fysikum(Swepub:su)mstoc
(författare)
-
Kjær, Christina
(författare)
-
Ashworth, Eleanor K.
(författare)
-
Bulman Page, Philip C.
(författare)
-
Meech, Stephen R.
(författare)
-
Brøndsted Nielsen, Steen
(författare)
-
Blancafort, Lluís
(författare)
-
Hopkins, W. Scott
(författare)
-
Bull, James N.
(författare)
-
Stockholms universitetFysikum
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal of Chemical Physics: AIP Publishing155:120021-96061089-7690
Internetlänk
Hitta via bibliotek
Till lärosätets databas