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Correlation of sist...
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Matsuoka, Atsuko
(författare)
Correlation of sister chromatid exchange formation through homologous recombination with ribonucleotide reductase inhibition.
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-20346
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-20346URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:vet swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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We conducted the recombination and sister chromatid exchange (SCE) assays with five chemicals (hydroxyurea (HU), resveratrol, 4-hydroxy-trans-stilbene, 3-hydroxy-trans-stilbene, and mitomycin C) in Chinese hamster cell line SPD8/V79 to confirm directly that SCE is a result of homologous recombination (HR). SPD8 has a partial duplication in exon 7 of the endogenous hprt gene and can revert to wild type by homologous recombination. All chemicals were positive in both assays except for 3-hydroxy-trans-stilbene, which was negative in both. HU, resveratrol, and 4-hydroxy-trans-stilbene were scavengers of the tyrosyl free radical of the R2 subunit of mammalian ribonucleotide reductase. Tyrosyl free radical scavengers disturb normal DNA replication, causing replication fork arrest. Mitomycin C is a DNA cross-linking agent that also causes replication fork arrest. The present study suggests that replication fork arrest, which is similar to the early phases of HR, leads to a high frequency of recombination, resulting in SCEs. The findings show that SCE may be mediated by HR.
Ämnesord och genrebeteckningar
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Alkylating Agents/toxicity
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Animals
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CHO Cells
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Cricetinae
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Cricetulus
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Crossing Over; Genetic/*drug effects
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DNA Replication
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Enzyme Inhibitors/toxicity
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Hydroxyurea/toxicity
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Mitomycin/toxicity
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Protein Subunits/drug effects
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Ribonucleotide Reductases/*antagonists & inhibitors/chemistry/drug effects
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Sister Chromatid Exchange
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Stilbenes/toxicity
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Structure-Activity Relationship
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Time Factors
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Lundin, Cecilia
(författare)
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Johansson, Fredrik
(författare)
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Sahlin, MargaretaStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik(Swepub:su)msahl
(författare)
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Fukuhara, Kiyoshi
(författare)
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Sjöberg, Britt-MarieStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik(Swepub:su)bsjob
(författare)
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Jenssen, Dag
(författare)
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Önfelt, Agneta
(författare)
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Stockholms universitetInstitutionen för molekylärbiologi och funktionsgenomik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Mutat Res547:1-2, s. 101-70027-5107
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