Mutant R1 proteins from Escherichia coli class Ia ribonucleotide reductase with altered responses to dATP inhibition.

• Aerobic ribonucleotide reductase from Escherichia coli regulates its level of activity by binding of effectors to an allosteric site in R1, located to the proposed interaction area of the two proteins that comprise the class I enzyme. Activity is increased by ATP binding and decreased by dATP binding. To study the mechanism governing this regulation, we have constructed three R1 proteins with mutations at His-59 in the activity site and one R1 protein with a mutation at His-88 close to the activity site and compared their allosteric behavior to that of the wild type R1 protein. All mutant proteins retained about 70% of wild type enzymatic activity. We found that if residue His-59 was replaced with alanine or asparagine, the enzyme lost its normal response to the inhibitory effect of dATP, whereas the enzyme with a glutamine still managed to elicit a normal response. We saw a similar result if residue His-88, which is proposed to hydrogen-bond to His-59, was replaced with alanine. Nucleotide binding experiments ruled out the possibility that the effect is due to an inability of the mutant proteins to bind effector since little difference in binding constants was observed for wild type and mutant proteins. Instead, the interaction between proteins R1 and R2 was perturbed in the mutant proteins. We propose that His-59 is important in the allosteric effect triggered by dATP binding, that the conserved hydrogen bond between His-59 and His-88 is important for the communication of the allosteric effect, and that this effect is exerted on the R1/R2 interaction.

Nyckelord

Adenosine Triphosphate/chemistry/*metabolism

Allosteric Site

Asparagine/chemistry

Binding Sites

Cell Division

Dose-Response Relationship; Drug

Escherichia coli/*genetics/metabolism

Glutamine/chemistry

Histidine/chemistry

Hydrogen Bonding

Kinetics

Magnesium/chemistry

Models; Molecular

Mutagenesis; Site-Directed

Mutation

Oligonucleotides/chemistry

Plasmids/metabolism

Protein Binding

Ribonucleotide Reductases/chemistry/*genetics

Substrate Specificity

Surface Plasmon Resonance

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