Sökning: onr:"swepub:oai:DiVA.org:su-20685" >
Co-transduction of ...
Co-transduction of Sleeping Beauty Transposase and Donor Plasmid via a Cell-penetrating Peptide : A simple one-step method
-
- Järver, Peter (författare)
- Stockholms universitet,Institutionen för neurokemi,University of Tartu, Estonia
-
- Fernaeus, Sandra (författare)
- Stockholms universitet,Institutionen för neurokemi
-
- EL Andaloussi, Samir (författare)
- Stockholms universitet,Institutionen för neurokemi
-
visa fler...
-
- Tjörnhammar, Marie-Louise (författare)
- Stockholms universitet,Institutionen för neurokemi
-
- Langel, Ülo (författare)
- Stockholms universitet,Institutionen för neurokemi
-
visa färre...
-
(creator_code:org_t)
- 2007-09-08
- 2008
- Engelska.
-
Ingår i: International journal of peptide research and therapeutics. - : Springer Science and Business Media LLC. - 1573-3904 .- 1573-3149. ; 14:1, s. 58-63
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Transposable elements have emerged as a promising candidate for human non-viral gene-therapy. The Tc1/mariner transposon Sleeping Beauty is to date one of the most efficient transposons in mammals. Sleeping Beauty transposase has so far mostly been delivered to cells via a DNA source. This might cause spontaneous integration of the transposase gene and cause fatal damage to the affected cell. Hence, it would be advantageous to employ a non-genetic source for the transposase. We here show that a novel Cell-penetrating peptide, M918, has the ability to facilitate cellular delivery of both the transposase Sleeping Beauty as a protein and a transposon donor-plasmid carrying an antibiotic resistance gene in vitro. The technique is a simple and straightforward one-step method that might render a safe and efficient delivery platform for Sleeping Beauty mediated gene therapy.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Cell-penetrating peptides
- Protein delivery
- Transfection
- Transposons
- Chemistry
- Kemi
- neurokemi med molekylär neurobiologi
- Neurochemistry with Molecular Neurobiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas