Sir2 and Reb1 antagonistically regulate nucleosome occupancy in subtelomeric X-elements and repress TERRAs by distinct mechanisms

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Bauer, Stefanie L.,1990-Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut (author)

Sir2 and Reb1 antagonistically regulate nucleosome occupancy in subtelomeric X-elements and repress TERRAs by distinct mechanisms

Article/chapterEnglish2022

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2022-09-22
Public Library of Science (PLoS),2022
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LIBRIS-ID:oai:DiVA.org:su-209902
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-209902URI
https://doi.org/10.1371/journal.pgen.1010419DOI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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Telomere chromatin structure is pivotal for maintaining genome stability by regulating the binding of telomere-associated proteins and inhibiting the DNA damage response. In Saccharomyces cerevisiae, silent information regulator (Sir) proteins bind to terminal repeats and to subtelomeric X-elements, resulting in transcriptional silencing. Herein, we show that sir2 mutant strains display a specific loss of a nucleosome residing in the X-elements and that this deficiency is remarkably consistent between different telomeres. The X-elements contain several binding sites for the transcription factor Reb1 and we found that Sir2 and Reb1 compete for stabilizing/destabilizing this nucleosome, i.e. inactivation of Reb1 in a sir2 background reinstated the lost nucleosome. The telomeric-repeat-containing RNAs (TERRAs) originate from subtelomeric regions and extend into the terminal repeats. Both Sir2 and Reb1 repress TERRAs and in a sir2 reb1 double mutant, TERRA levels increased synergistically, showing that Sir2 and Reb1 act in different pathways for repressing TERRAs. We present evidence that Reb1 restricts TERRAs by terminating transcription. Mapping the 5′-ends of TERRAs from several telomeres revealed that the Sir2-stabilized nucleosome is the first nucleosome downstream from the transcriptional start site for TERRAs. Finally, moving an X-element to a euchromatic locus changed nucleosome occupancy and positioning, demonstrating that X-element nucleosome structure is dependent on the local telomere environment.

Subject headings and genre

NATURVETENSKAP Biologi Genetik hsv//swe
NATURAL SCIENCES Biological Sciences Genetics hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Utvecklingsbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Developmental Biology hsv//eng

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Thaisen Grochalski, Thomas Nagel,1989-Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)thgr4979 (author)
Smialowska, AgataStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)asmia (author)
Åström, Stefan U.Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)stast (author)
Stockholms universitetInstitutionen för molekylär biovetenskap, Wenner-Grens institut (creator_code:org_t)

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In:PLOS Genetics: Public Library of Science (PLoS)18:91553-73901553-7404

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Genetics

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