Search: WFRF:(Zayed A. A.) >
Structural Diversit...
Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract
-
- Zayed, Muhammad (author)
- Menoufia Univ, Fac Sci, Dept Bot & Microbiol, Menoufia 32511, Egypt.
-
- El-Garawani, Islam M. (author)
- Menoufia Univ, Fac Sci, Dept Zool, Menoufia 32511, Egypt.
-
- El-Sabbagh, Sabha M. (author)
- Menoufia Univ, Fac Sci, Dept Bot & Microbiol, Menoufia 32511, Egypt.
-
show more...
-
- Amr, Bassem (author)
- Menoufia Univ, Fac Sci, Dept Bot & Microbiol, Menoufia 32511, Egypt.
-
- Alsharif, Sultan M. (author)
- Taibah Univ, Fac Sci, Biol Dept, Al Madinah 887, Saudi Arabia.
-
- Tayel, Ahmed A. (author)
- Kafrelsheikh Univ, Fac Aquat & Fisheries Sci, Dept Fish Proc & Biotechnol, Kafrelsheikh 33516, Egypt.
-
- AlAjmi, Mohamed F. (author)
- King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia.
-
- Ibrahim, Hasnaa M. S. (author)
- Menoufia Univ, Fac Sci, Dept Chem, Menoufia 32511, Egypt.
-
- Shou, Qiyang (author)
- Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou 310058, Peoples R China.
-
- Khalifa, Shaden A. M. (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, S-10691 Stockholm, Sweden.
-
- El-Seedi, Hesham (author)
- Uppsala universitet,Farmakognosi,Institutionen för farmaceutisk biovetenskap,Menoufia Univ, Fac Sci, Dept Chem, Menoufia 32511, Egypt.;Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Peoples R China.;Jiangsu Univ, Jiangsu Educ Dept, Int Joint Res Lab Intelligent Agr & Agri Prod Proc, Nanjing 210024, Peoples R China.
-
- Elfeky, Nora (author)
- Menoufia Univ, Fac Sci, Dept Bot & Microbiol, Menoufia 32511, Egypt.
-
show less...
-
Menoufia Univ, Fac Sci, Dept Bot & Microbiol, Menoufia 32511, Egypt Menoufia Univ, Fac Sci, Dept Zool, Menoufia 32511, Egypt. (creator_code:org_t)
- 2022-11-11
- 2022
- English.
-
In: Metabolites. - : MDPI AG. - 2218-1989 .- 2218-1989. ; 12:11
- Related links:
-
https://doi.org/10.3...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial strains. A bacterial isolate (BF202) cultured from the marine sediments of Alexandria’s Mariout Lake (Egypt) was tested for its antimicrobial and anticancer potential. The phylogenetic analysis of the isolated strain’s 16S rDNA and gyrB revealed that BF202 belongs to Brevibacillus laterosporus (B. laterosporus). Antibiosis of B. laterosporus was confirmed against microbial pathogens including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Staphylococcus aureus. The highest antibacterial activity was detected on glucose peptone medium after 18 h of incubation at 35 °C, and at pH of 7.0 in the presence of mannose and ammonium carbonate as carbon and nitrogen sources, respectively. The cytotoxicity of the methanolic extract against breast cancer (MCF-7) and normal Vero cell lines, using the MTT test, revealed IC50 values of 7.93 and 23.79 µg/mL, respectively. To identify apoptotic and necrotic cells, a flow cytometric analysis using annexin V-FITC/PI dual-labeling was utilized and recorded a higher number of necrotic cells compared to apoptotic ones. Similarly, the cell cycle S-phase arrest was reported. The LC-MS-MS investigation of B. laterosporus extract and the molecular networking database analysis demonstrated five strategic diketopiperazine compounds with antimicrobial and anticancer activities. Taken together, this research shows that the crude extract of B. laterosporus might be an effective agent against drug-resistant bacteria and malignant disorders due to its richness in diketopiperazines.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Brevibacillus laterosporus
- marine sediment
- cyclo dipeptides
- cytotoxicity
- antimicrobial
- phylogenetic analysis
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Zayed, Muhammad
-
El-Garawani, Isl ...
-
El-Sabbagh, Sabh ...
-
Amr, Bassem
-
Alsharif, Sultan ...
-
Tayel, Ahmed A.
-
show more...
-
AlAjmi, Mohamed ...
-
Ibrahim, Hasnaa ...
-
Shou, Qiyang
-
Khalifa, Shaden ...
-
El-Seedi, Hesham
-
Elfeky, Nora
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Microbiology
- Articles in the publication
-
Metabolites
- By the university
-
Stockholm University
-
Uppsala University